Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ABDX87_RS16000 Genome accession   NZ_CP155619
Coordinates   3584441..3585505 (+) Length   354 a.a.
NCBI ID   WP_074751550.1    Uniprot ID   -
Organism   Pseudomonas abietaniphila strain BHJ04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3579441..3590505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABDX87_RS15990 (ABDX87_15990) - 3580345..3581613 (+) 1269 WP_346828758.1 malic enzyme-like NAD(P)-binding protein -
  ABDX87_RS15995 (ABDX87_15995) - 3581758..3584235 (-) 2478 WP_346828759.1 penicillin-binding protein 1A -
  ABDX87_RS16000 (ABDX87_16000) pilM 3584441..3585505 (+) 1065 WP_074751550.1 pilus assembly protein PilM Machinery gene
  ABDX87_RS16005 (ABDX87_16005) - 3585505..3586071 (+) 567 WP_346828760.1 PilN domain-containing protein -
  ABDX87_RS16010 (ABDX87_16010) pilO 3586068..3586715 (+) 648 WP_346828761.1 type 4a pilus biogenesis protein PilO -
  ABDX87_RS16015 (ABDX87_16015) pilP 3586712..3587239 (+) 528 WP_346828762.1 type 4a pilus biogenesis lipoprotein PilP -
  ABDX87_RS16020 (ABDX87_16020) pilQ 3587293..3589383 (+) 2091 WP_346828763.1 type IV pilus secretin PilQ Machinery gene
  ABDX87_RS16025 (ABDX87_16025) aroK 3589388..3589906 (+) 519 WP_346828764.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38188.76 Da        Isoelectric Point: 4.6991

>NTDB_id=998041 ABDX87_RS16000 WP_074751550.1 3584441..3585505(+) (pilM) [Pseudomonas abietaniphila strain BHJ04]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGNALTRVLVKAKSNVKIAAV
AVAGSAVITKTIEMDAGLSDDEMENQLKIEADQYIPYPLEEVAIDFEVQGYSARNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLSAQLGQDHEQLTVAVIDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLSV
GEAGLAKKQGGLPDDYVREVLQPFKDAVVQQVSRSLQFFFAAGQYNRVDYIMLAGGTASIAGLDRLIQERLGTPTMVANP
FADMALSAKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=998041 ABDX87_RS16000 WP_074751550.1 3584441..3585505(+) (pilM) [Pseudomonas abietaniphila strain BHJ04]
GTGTTCGAACTCTTCAGTAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTTGAATT
GAGTCGTTCCGGTACCCGCTACAAGGTCGAGTCTTACGCGGTAGAACCCCTCCCCGCCAACGCGGTGGTCGAAAAGAACA
TTGCCGAGCTTGAAGGCGTCGGCAATGCGTTGACGCGCGTGCTGGTCAAGGCCAAGAGTAACGTCAAAATCGCGGCGGTC
GCCGTCGCGGGTTCGGCAGTCATTACAAAGACCATCGAGATGGACGCCGGTCTTTCCGACGACGAGATGGAAAACCAGCT
CAAGATCGAGGCCGACCAGTACATTCCCTATCCGCTTGAAGAAGTCGCCATCGACTTCGAGGTGCAGGGGTACTCCGCGC
GTAACCCAGAGCGCGTTGAAGTCCTGCTTGCCGCTTGCCGCAAGGAAAACGTCGAAGTCCGCGAAGCGGCGCTGGCGCTG
GCCGGTCTAACGGCACGCGTGGTCGATGTCGAAGCCTATGCGCTGGAACGCTCCTTTGGCCTGCTCTCGGCTCAGCTCGG
GCAGGATCACGAACAATTGACGGTGGCCGTCATCGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACAATGGCC
GCATCATCTATACCCGCGAACAGCTGTTCGGCGGGCGTCAGTTGACCGAAGAAATCCAGCGTCGCTATGGCTTGTCGGTG
GGCGAAGCGGGGCTTGCGAAGAAGCAGGGCGGGCTGCCGGATGACTATGTGCGCGAGGTCCTGCAACCCTTCAAAGATGC
CGTGGTTCAGCAGGTTTCCCGTTCGCTGCAATTCTTCTTCGCGGCCGGCCAGTACAACCGTGTCGACTACATCATGCTGG
CGGGCGGGACGGCGTCCATTGCAGGTCTTGATCGCTTGATCCAGGAGCGCCTGGGCACGCCAACCATGGTGGCTAACCCG
TTTGCCGACATGGCGTTGAGCGCCAAGGTCAACGCCGGTGCGCTGGCCAGCGATGCGCCGGCATTGATGATCGCGTGCGG
TCTGGCTTTGAGGAGCTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466

Multiple sequence alignment