Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SriyCCGPB3_RS04815 Genome accession   NZ_CP155568
Coordinates   1030612..1031775 (+) Length   387 a.a.
NCBI ID   WP_049400675.1    Uniprot ID   A0AA42HYU0
Organism   Stenotrophomonas riyadhensis strain ESTM1A_MKCTX4_10a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1025612..1036775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SriyCCGPB3_RS04785 (SriyCCGPB3_000956) soxR 1025744..1026178 (+) 435 WP_094002028.1 redox-sensitive transcriptional activator SoxR -
  SriyCCGPB3_RS04790 (SriyCCGPB3_000957) - 1026252..1026854 (-) 603 WP_094002029.1 short chain dehydrogenase -
  SriyCCGPB3_RS04795 (SriyCCGPB3_000958) - 1026955..1027830 (+) 876 WP_153856478.1 LysR family transcriptional regulator -
  SriyCCGPB3_RS04800 (SriyCCGPB3_000959) proC 1027891..1028712 (-) 822 WP_153856477.1 pyrroline-5-carboxylate reductase -
  SriyCCGPB3_RS04805 (SriyCCGPB3_000960) - 1028731..1029408 (-) 678 WP_324187345.1 YggS family pyridoxal phosphate-dependent enzyme -
  SriyCCGPB3_RS04810 (SriyCCGPB3_000961) pilT 1029496..1030533 (+) 1038 WP_006422551.1 type IV pilus twitching motility protein PilT Machinery gene
  SriyCCGPB3_RS04815 (SriyCCGPB3_000962) pilU 1030612..1031775 (+) 1164 WP_049400675.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SriyCCGPB3_RS04820 (SriyCCGPB3_000963) - 1031898..1032539 (+) 642 WP_153856476.1 hypothetical protein -
  SriyCCGPB3_RS04825 (SriyCCGPB3_000964) - 1032651..1033634 (+) 984 WP_153856475.1 aldo/keto reductase -
  SriyCCGPB3_RS04830 (SriyCCGPB3_000965) - 1033701..1034600 (-) 900 WP_110711393.1 LysR family transcriptional regulator -
  SriyCCGPB3_RS04835 (SriyCCGPB3_000966) - 1034903..1035115 (+) 213 WP_005408372.1 DUF4287 domain-containing protein -
  SriyCCGPB3_RS04840 (SriyCCGPB3_000967) - 1035211..1035660 (+) 450 WP_153856474.1 GNAT family N-acetyltransferase -
  SriyCCGPB3_RS04845 (SriyCCGPB3_000968) - 1035754..1036461 (+) 708 WP_088028269.1 YitT family protein -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43172.60 Da        Isoelectric Point: 6.6615

>NTDB_id=997809 SriyCCGPB3_RS04815 WP_049400675.1 1030612..1031775(+) (pilU) [Stenotrophomonas riyadhensis strain ESTM1A_MKCTX4_10a]
MPHWEPAVNTTATTIDFTSFLKLMAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKT
HECNFAIGLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGY
RNQNSTGHIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLC
TLHANNANQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVM
KDSVQLGMKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=997809 SriyCCGPB3_RS04815 WP_049400675.1 1030612..1031775(+) (pilU) [Stenotrophomonas riyadhensis strain ESTM1A_MKCTX4_10a]
ATGCCCCATTGGGAGCCCGCCGTGAACACCACCGCGACCACCATCGATTTCACTTCGTTCCTCAAGCTGATGGCGCACCA
GCGCGCCTCCGACCTGTTCATCACCGCAGGCATGCCGCCGGCGATGAAAGTCAACGGCAAGATCTCGCCGATCACGCAGA
CCCCGCTCACGCCGCAGCAGAGCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACC
CACGAATGCAACTTCGCCATCGGCCTGTCCGGTGTCGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTCGG
CATGGTGCTGCGTCGCATCGAGACGCGCATTCCGACCGTGGAAGAGCTGAGCCTGCCGCCGATCATCAAGACGCTGGCGA
TGACCAAGCGCGGCATCATCCTGTTCGTCGGCGCCACCGGTACCGGTAAGTCGACCTCGCTGGCAGCGATGATCGGCTAC
CGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCAT
CACCCAGCGCGAAGTCGGCATCGATACCGACAGTTGGGAAGCGGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGA
TCATGATCGGCGAGGTGCGTACCCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAAACCGGCCACCTGGTGCTGTGC
ACCCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGCAACCAGCTGCT
GATGGACCTGTCGCTGAACCTCAAGGGCGTGGTCGCGCAGCAGCTGGTGCCGTCGCCCGATGGCCGCTCGCGCAAGGTGG
CGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATG
AAGGACTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCGGGCGAGATCAGCTACGA
GGACGCGCTGCGCTACGCCGATTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGACGCGCGCACCC
TGTCGCAGGGGCTGGACGGGGTGGAGATCTCCGAGATCCGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.847

94.832

0.643
  pilU Acinetobacter baylyi ADP1

63.585

92.248

0.587
  pilU Vibrio cholerae strain A1552

53.371

91.99

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.099

91.731

0.395

Multiple sequence alignment