Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   EL041_RS02025 Genome accession   NZ_LR134092
Coordinates   437776..439014 (+) Length   412 a.a.
NCBI ID   WP_000815965.1    Uniprot ID   -
Organism   Escherichia coli strain NCTC10444     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 432776..444014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL041_RS02000 (NCTC10444_00395) mrcA 433056..435608 (-) 2553 WP_001301905.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  EL041_RS02005 (NCTC10444_00396) hofM 435728..436507 (+) 780 WP_024257791.1 DNA utilization protein HofM -
  EL041_RS02010 (NCTC10444_00397) hofN 436507..437046 (+) 540 WP_001069348.1 DNA utilization protein HofN -
  EL041_RS02015 (NCTC10444_00398) hofO 437030..437470 (+) 441 WP_001055765.1 DNA utilization protein HofO -
  EL041_RS02020 (NCTC10444_00399) hofP 437460..437864 (+) 405 WP_001264139.1 DNA utilization protein HofP -
  EL041_RS02025 (NCTC10444_00400) comE 437776..439014 (+) 1239 WP_000815965.1 DNA uptake porin HofQ Machinery gene
  EL041_RS02030 (NCTC10444_00401) aroK 439415..439936 (+) 522 WP_000818618.1 shikimate kinase AroK -
  EL041_RS02035 (NCTC10444_00402) aroB 439993..441081 (+) 1089 WP_000439848.1 3-dehydroquinate synthase -
  EL041_RS02040 (NCTC10444_00403) damX 441173..442459 (+) 1287 WP_000343210.1 cell division protein DamX -
  EL041_RS02045 (NCTC10444_00404) dam 442566..443402 (+) 837 WP_000742147.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44735.24 Da        Isoelectric Point: 5.9488

>NTDB_id=997527 EL041_RS02025 WP_000815965.1 437776..439014(+) (comE) [Escherichia coli strain NCTC10444]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTIVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=997527 EL041_RS02025 WP_000815965.1 437776..439014(+) (comE) [Escherichia coli strain NCTC10444]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTATAGTGAAAAGCGCCGGACTG
ATAACGCGCCAGGAGGGCAACATTCTCTCAGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGTCAGGAAGCGGA
GCAGGCGCGGGCGCAGGCAAATCTACCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCTGGGGAAAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCGCACTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGTTTGCTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAAGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCAGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTGCCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae 86-028NP

37.529

100

0.398
  comE Haemophilus influenzae Rd KW20

37.385

100

0.396
  pilQ Vibrio campbellii strain DS40M4

38.48

100

0.393
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

38.015

100

0.381
  pilQ Vibrio cholerae strain A1552

38.015

100

0.381
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364

Multiple sequence alignment