Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   ABC814_RS10280 Genome accession   NZ_CP155533
Coordinates   1977425..1977886 (-) Length   153 a.a.
NCBI ID   WP_000250520.1    Uniprot ID   C1CA95
Organism   Streptococcus pneumoniae strain Spn1439-106     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1972425..1982886
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABC814_RS10260 (ABC814_10260) comYH 1973651..1974604 (-) 954 WP_000345132.1 class I SAM-dependent methyltransferase Machinery gene
  ABC814_RS10265 (ABC814_10265) - 1974950..1975752 (+) 803 Protein_1998 IS5 family transposase -
  ABC814_RS10270 (ABC814_10270) - 1975903..1976379 (+) 477 WP_000777686.1 GNAT family N-acetyltransferase -
  ABC814_RS10275 (ABC814_10275) comGG/cglG 1977034..1977447 (-) 414 WP_000265615.1 competence type IV pilus minor pilin ComGG Machinery gene
  ABC814_RS10280 (ABC814_10280) comGF/cglF 1977425..1977886 (-) 462 WP_000250520.1 competence type IV pilus minor pilin ComGF Machinery gene
  ABC814_RS10285 (ABC814_10285) comGE/cglE 1977849..1978151 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  ABC814_RS10290 (ABC814_10290) comGD/cglD 1978114..1978548 (-) 435 WP_033606693.1 competence type IV pilus minor pilin ComGD Machinery gene
  ABC814_RS10295 (ABC814_10295) comGC/cglC 1978511..1978834 (-) 324 WP_000738628.1 comG operon protein ComGC Machinery gene
  ABC814_RS10300 (ABC814_10300) comGB/cglB 1978836..1979852 (-) 1017 WP_078141518.1 competence type IV pilus assembly protein ComGB Machinery gene
  ABC814_RS10305 (ABC814_10305) comGA/cglA/cilD 1979800..1980741 (-) 942 WP_000249572.1 competence type IV pilus ATPase ComGA Machinery gene
  ABC814_RS10310 (ABC814_10310) - 1980817..1981182 (-) 366 WP_000286415.1 DUF1033 family protein -
  ABC814_RS10315 (ABC814_10315) - 1981333..1982391 (-) 1059 WP_000649465.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17905.50 Da        Isoelectric Point: 8.0031

>NTDB_id=997200 ABC814_RS10280 WP_000250520.1 1977425..1977886(-) (comGF/cglF) [Streptococcus pneumoniae strain Spn1439-106]
MVQNSCWQSKSHKVKAFTLLESLIALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKEIAMGKSKADDFRKTNARGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide


Download         Length: 462 bp        

>NTDB_id=997200 ABC814_RS10280 WP_000250520.1 1977425..1977886(-) (comGF/cglF) [Streptococcus pneumoniae strain Spn1439-106]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGATTGCCCTGAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGAGATTGCTATGGGTAAGTCTAAGGCAGATGATTTCCGTAAAACGAATGCTCG
TGGTCGAGGTTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C1CA95

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae D39

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae R6

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae TIGR4

93.464

100

0.935
  comGF/cglF Streptococcus mitis NCTC 12261

90.85

100

0.909
  comGF/cglF Streptococcus mitis SK321

90.85

100

0.909

Multiple sequence alignment