Detailed information NTG0995782

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M9Y83_RS02120
• Genome accession: NZ_CP155048
• Coordinates: 450263..451411 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain CCBH26428
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 445263..456411

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M9Y83_RS02095 (M9Y83_0002095)	-	446414..446710 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
M9Y83_RS02100 (M9Y83_0002100)	-	446707..447300 (-)	594	WP_003084544.1	YggT family protein	-
M9Y83_RS02105 (M9Y83_0002105)	proC	447311..448132 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
M9Y83_RS02110 (M9Y83_0002110)	-	448144..448836 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
M9Y83_RS02115 (M9Y83_0002115)	pilT	449051..450085 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
M9Y83_RS02120 (M9Y83_0002120)	pilU	450263..451411 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
M9Y83_RS02125 (M9Y83_0002125)	czcD	451419..452318 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
M9Y83_RS02130 (M9Y83_0002130)	-	452472..452876 (+)	405	WP_003084560.1	NINE protein	-
M9Y83_RS02135 (M9Y83_0002135)	-	453119..454492 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
M9Y83_RS02140 (M9Y83_0002140)	-	454489..455673 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=995782 M9Y83_RS02120 WP_003100959.1 450263..451411(+) (pilU) [Pseudomonas aeruginosa strain CCBH26428]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=995782 M9Y83_RS02120 WP_003100959.1 450263..451411(+) (pilU) [Pseudomonas aeruginosa strain CCBH26428]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382