Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   AAYR29_RS03235 Genome accession   NZ_CP154918
Coordinates   555930..556547 (+) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis strain FUA2231     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 550930..561547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAYR29_RS03200 (AAYR29_03200) aroE 551594..552436 (+) 843 WP_038429328.1 shikimate dehydrogenase -
  AAYR29_RS03205 (AAYR29_03205) yhbY 552430..552720 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  AAYR29_RS03210 (AAYR29_03210) nadD 552732..553301 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  AAYR29_RS03215 (AAYR29_03215) yqeK 553291..553851 (+) 561 WP_029317884.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  AAYR29_RS03220 (AAYR29_03220) rsfS 553869..554219 (+) 351 WP_345806061.1 ribosome silencing factor -
  AAYR29_RS03225 (AAYR29_03225) yqeM 554216..554959 (+) 744 WP_106073582.1 class I SAM-dependent methyltransferase -
  AAYR29_RS03230 (AAYR29_03230) comER 555025..555846 (-) 822 WP_004398597.1 late competence protein ComER -
  AAYR29_RS03235 (AAYR29_03235) comEA 555930..556547 (+) 618 WP_004398514.1 competence protein ComEA Machinery gene
  AAYR29_RS03240 (AAYR29_03240) comEB 556614..557183 (+) 570 WP_015714288.1 ComE operon protein 2 -
  AAYR29_RS03245 (AAYR29_03245) comEC 557180..559510 (+) 2331 WP_345806062.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  AAYR29_RS03250 (AAYR29_03250) yqzM 559550..559684 (-) 135 WP_003229983.1 YqzM family protein -
  AAYR29_RS03255 (AAYR29_03255) - 559725..559874 (+) 150 WP_003229985.1 hypothetical protein -
  AAYR29_RS03260 (AAYR29_03260) holA 559914..560957 (+) 1044 WP_345806063.1 DNA polymerase III subunit delta -
  AAYR29_RS03265 (AAYR29_03265) rpsT 560972..561238 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=995350 AAYR29_RS03235 WP_004398514.1 555930..556547(+) (comEA) [Bacillus subtilis strain FUA2231]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=995350 AAYR29_RS03235 WP_004398514.1 555930..556547(+) (comEA) [Bacillus subtilis strain FUA2231]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment