Detailed information NTG0994704

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AAVD18_RS08745
• Genome accession: NZ_CP154893
• Coordinates: 1663391..1664005 (-)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus amyloliquefaciens strain Fad 11/2
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1658391..1669005

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAVD18_RS08715 (AAVD18_08715)	rpsT	1658691..1658957 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
AAVD18_RS08720 (AAVD18_08720)	holA	1658974..1660015 (-)	1042	Protein_1718	DNA polymerase III subunit delta	-
AAVD18_RS08725 (AAVD18_08725)	-	1660055..1660209 (-)	155	Protein_1719	hypothetical protein	-
AAVD18_RS08730 (AAVD18_08730)	-	1660250..1660384 (+)	135	WP_003152870.1	YqzM family protein	-
AAVD18_RS08735 (AAVD18_08735)	comEC	1660403..1662754 (-)	2352	WP_041481655.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AAVD18_RS08740 (AAVD18_08740)	-	1662755..1663324 (-)	570	WP_003152868.1	ComE operon protein 2	-
AAVD18_RS08745 (AAVD18_08745)	comEA	1663391..1664005 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
AAVD18_RS08750 (AAVD18_08750)	comER	1664064..1664885 (+)	822	WP_013352943.1	late competence protein ComER	-
AAVD18_RS08755 (AAVD18_08755)	-	1664954..1665691 (-)	738	WP_013352944.1	class I SAM-dependent methyltransferase	-
AAVD18_RS08760 (AAVD18_08760)	rsfS	1665688..1666044 (-)	357	WP_003152864.1	ribosome silencing factor	-
AAVD18_RS08765 (AAVD18_08765)	yqeK	1666062..1666622 (-)	561	WP_013352945.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AAVD18_RS08770 (AAVD18_08770)	-	1666612..1667181 (-)	570	WP_013352946.1	nicotinate-nucleotide adenylyltransferase	-
AAVD18_RS08775 (AAVD18_08775)	yhbY	1667192..1667482 (-)	291	WP_061573777.1	ribosome assembly RNA-binding protein YhbY	-
AAVD18_RS08780 (AAVD18_08780)	aroE	1667476..1668318 (-)	843	WP_013352947.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=994704 AAVD18_RS08745 WP_013352942.1 1663391..1664005(-) (comEA) [Bacillus amyloliquefaciens strain Fad 11/2]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=994704 AAVD18_RS08745 WP_013352942.1 1663391..1664005(-) (comEA) [Bacillus amyloliquefaciens strain Fad 11/2]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCCT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363