Detailed information NTG0994375

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: EW023_RS00340
• Genome accession: NZ_LR130239
• Coordinates: 54009..55007 (+)
• Length: 332 a.a.
• NCBI ID: WP_002986679.1
• Uniprot ID: A2RC05
• Organism: Streptococcus pyogenes strain PS003 isolate PS003
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 49009..60007

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EW023_RS00325	-	50012..51430 (+)	1419	WP_023612101.1	hypothetical protein	-
EW023_RS00330	purB	51450..52742 (+)	1293	WP_023612104.1	adenylosuccinate lyase	-
EW023_RS00335	comR	52873..53784 (+)	912	WP_002986681.1	transcriptional regulator Rgg4/ComR	Regulator
EW023_RS10010	comS	53864..53962 (+)	99	WP_206733287.1	quorum-sensing system DWW-type pheromone	Regulator
EW023_RS00340	ruvB	54009..55007 (+)	999	WP_002986679.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
EW023_RS00345	-	55145..55582 (+)	438	WP_002994668.1	low molecular weight protein-tyrosine-phosphatase	-
EW023_RS00350	-	55605..56006 (+)	402	WP_002986671.1	MORN repeat-containing protein	-
EW023_RS00355	-	56003..57778 (+)	1776	WP_129758815.1	acyltransferase family protein	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37565.11 Da
• Isoelectric Point: 5.3005

>NTDB_id=994375 EW023_RS00340 WP_002986679.1 54009..55007(+) (ruvB) [Streptococcus pyogenes strain PS003 isolate PS003]
MARILDNNVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEAAKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAVEKAGDLVAILNELEPGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPRIANRLLKRVRDYAQIIGDGIIT
AQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGGPVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQK
AYRHLGYPYQNT

Nucleotide

• Length: 999 bp

>NTDB_id=994375 EW023_RS00340 WP_002986679.1 54009..55007(+) (ruvB) [Streptococcus pyogenes strain PS003 isolate PS003]
ATGGCTAGAATTTTAGACAATAATGTAATGGGAAATGAGGAGTTTTCGGATCGGACTCTTCGCCCTCAATATCTACACGA
ATATATTGGACAAGATAAAGTTAAGGAACAATTCGCTATTTTTATAGAAGCAGCTAAAAGACGTGACGAATCTTTAGACC
ATGTTTTATTATTTGGACCTCCAGGACTGGGAAAAACAACAATGGCATTTGTGATTGCCAATGAATTGGGAGTTAATTTA
AAGCAAACTTCAGGTCCTGCCGTTGAGAAAGCTGGAGATTTGGTAGCTATTTTAAATGAATTAGAACCAGGGGATATTTT
GTTTATTGATGAAATTCACCGCATGCCAATGTCTGTCGAGGAAGTACTATATAGTGCTATGGAAGACTTCTATATTGATA
TTATGATTGGCGCTGGAGATACTAGTCGTAGTATTCATTTGGATCTGCCTCCATTTACATTGATTGGTGCTACTACACGT
GCAGGCATGTTATCTAATCCCCTCAGAGCTCGTTTTGGTATCACTGGTCACATGGAATACTATCAAGAAAAGGATTTGAC
AGAGATTGTGGAGCGTACAGCTACTATTTTTGAAATAAAAATAGACCATGAAGCTGCACGGAAATTAGCTTGTCGCAGTA
GAGGAACGCCCCGCATTGCTAATCGATTACTAAAACGAGTGCGTGATTATGCTCAAATTATTGGTGATGGCATCATAACG
GCTCAAATAACTGATAGAGCTTTGACTATGTTAGATGTTGACCGCGAAGGTCTCGATTACATTGATCAGAAAATTTTACG
GACGATGATTGAGATGTATCAGGGAGGGCCAGTTGGTTTGGGGACATTATCTGTGAATATTGCCGAGGAGCGTAATACAG
TTGAAGAGATGTATGAACCCTACTTAATTCAAAAAGGCTTTCTCATGCGAACACGAACAGGTCGTGTAGCGACGCAAAAG
GCTTATCGTCATCTTGGCTATCCCTATCAAAACACTTAA

Domains

Predicted by InterProScan.

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: A2RC05

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae D39	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae TIGR4	84.545	99.398	0.84
ruvB	Bacillus subtilis subsp. subtilis str. 168	58.824	97.289	0.572
ruvB	Helicobacter pylori 26695	48.936	99.096	0.485
ruvB	Synechocystis sp. PCC 6803	50.16	94.277	0.473