Detailed information NTG0994312

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AAG092_RS01420
• Genome accession: NZ_CP154874
• Coordinates: 281506..282708 (+)
• Length: 400 a.a.
• NCBI ID: WP_373388212.1
• Uniprot ID: -
• Organism: Pseudomonas alcaligenes strain Med1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 276506..287708

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAG092_RS01390 (AAG092_01375)	-	276906..277739 (+)	834	WP_110683094.1	undecaprenyl-diphosphate phosphatase	-
AAG092_RS01395 (AAG092_01380)	-	277739..278419 (+)	681	WP_373388209.1	DUF1294 domain-containing protein	-
AAG092_RS01400 (AAG092_01385)	-	278416..278754 (-)	339	WP_373388210.1	MmcQ/YjbR family DNA-binding protein	-
AAG092_RS01405 (AAG092_01390)	-	278831..279337 (+)	507	WP_110683097.1	DUF1993 family protein	-
AAG092_RS01410 (AAG092_01395)	-	279392..280978 (-)	1587	WP_110683098.1	ABC-F family ATPase	-
AAG092_RS01415 (AAG092_01400)	-	281082..281360 (-)	279	WP_373388211.1	peptidylprolyl isomerase	-
AAG092_RS01420 (AAG092_01405)	pilU	281506..282708 (+)	1203	WP_373388212.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AAG092_RS01425 (AAG092_01410)	-	282734..284986 (-)	2253	WP_373388213.1	heavy metal translocating P-type ATPase	-

Sequence

Protein

• Length: 400 a.a.
• Molecular weight: 44244.66 Da
• Isoelectric Point: 6.1345

>NTDB_id=994312 AAG092_RS01420 WP_373388212.1 281506..282708(+) (pilU) [Pseudomonas alcaligenes strain Med1]
MDLTAMLKILSSQDGSDLYLSTGAPPCAKFNGVLKPLSQEPLKPGDVAAIANGVMDAEQRAEFDKELEMNLAISVPNVGR
FRINIFKQRNEVSIVARNIKMEIPKFEDLKLPEVLLKTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLRAFVSQRLVKTLDGKRRAAVEVLLGTPTIRDLIKRNEFSEIKEIMEKSKNLGMQTFDMAL
IDLVHEGAIDEEEAVKNADSANNVRLKLKLYKDNPASQSAPAPAAAPETKPAAAANWAMELKLEEIEEENPPEDPGRPGI

Nucleotide

• Length: 1203 bp

>NTDB_id=994312 AAG092_RS01420 WP_373388212.1 281506..282708(+) (pilU) [Pseudomonas alcaligenes strain Med1]
ATGGACCTCACCGCAATGCTGAAAATCCTGTCCAGCCAGGACGGGTCCGACCTCTACCTGTCCACCGGGGCGCCGCCCTG
CGCCAAGTTCAACGGGGTGCTCAAGCCCCTCAGCCAGGAGCCGCTCAAGCCGGGGGATGTGGCCGCTATCGCCAATGGCG
TGATGGATGCCGAGCAGCGCGCCGAGTTCGACAAGGAGCTGGAGATGAACCTGGCCATCTCGGTGCCCAACGTCGGTCGC
TTCCGCATCAACATCTTCAAGCAGCGCAACGAGGTGTCCATCGTCGCGCGCAACATCAAGATGGAGATTCCCAAGTTCGA
GGACCTCAAGCTGCCCGAGGTACTGCTCAAGACGGTGATGGAGAAGCGCGGTCTGGTGCTGTTCGTCGGCGGCACCGGCT
CCGGCAAGTCGACCTCGCTGGCGGCGCTGATCGACTACCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAC
CCGGTGGAGTATGTGCACCGGCACAAGAAATCGATCATCAACCAGCGCGAAGTCGGGGTCGACACCCGCAGCTTCCATGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGCACG
CCCTGGCCTTCGCGGACACCGGCCACCTGGCCATTTCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGTCGCCCACAGCTGCTCAATGACCTGGGCAACAACCTGCGCGCCTTCGTCTCGCAGCG
CCTGGTCAAGACCCTGGACGGCAAACGCCGTGCGGCGGTGGAAGTCCTGCTGGGCACGCCGACGATCCGCGACCTGATCA
AGCGCAACGAATTTTCCGAGATCAAGGAGATCATGGAGAAGTCGAAGAACCTCGGCATGCAGACCTTCGACATGGCGCTG
ATCGACCTGGTCCACGAGGGTGCCATCGACGAGGAGGAGGCGGTGAAGAATGCCGACTCGGCGAACAACGTCCGCCTCAA
GCTGAAACTCTACAAGGACAACCCGGCCAGCCAGTCGGCTCCGGCGCCCGCGGCGGCTCCCGAGACCAAGCCTGCCGCCG
CAGCCAACTGGGCGATGGAGCTCAAGCTCGAGGAGATCGAAGAGGAAAACCCGCCGGAAGATCCCGGTCGACCCGGCATC
TGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.41	87.75	0.495
pilU	Vibrio cholerae strain A1552	52	87.5	0.455
pilU	Acinetobacter baylyi ADP1	51.42	88	0.452
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.947	95	0.37