Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AAG895_RS14990 Genome accession   NZ_CP154859
Coordinates   3302293..3303144 (+) Length   283 a.a.
NCBI ID   WP_345792788.1    Uniprot ID   -
Organism   Thauera sp. JM12B12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3297293..3308144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG895_RS14975 (AAG895_14975) - 3297960..3299240 (+) 1281 WP_345792785.1 HlyC/CorC family transporter -
  AAG895_RS14980 (AAG895_14980) pilB 3299329..3301044 (+) 1716 WP_345792786.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAG895_RS14985 (AAG895_14985) pilC 3301060..3302292 (+) 1233 WP_345792787.1 type II secretion system F family protein Machinery gene
  AAG895_RS14990 (AAG895_14990) pilD 3302293..3303144 (+) 852 WP_345792788.1 A24 family peptidase Machinery gene
  AAG895_RS14995 (AAG895_14995) - 3303276..3303551 (+) 276 WP_345792789.1 zinc ribbon domain-containing protein -
  AAG895_RS15000 (AAG895_15000) - 3303553..3304176 (+) 624 WP_345792790.1 DUF502 domain-containing protein -
  AAG895_RS15005 (AAG895_15005) aspS 3304247..3306046 (+) 1800 WP_345792791.1 aspartate--tRNA ligase -
  AAG895_RS15010 (AAG895_15010) - 3306282..3307928 (+) 1647 WP_345792792.1 response regulator -

Sequence

Protein


Download         Length: 283 a.a.        Molecular weight: 30445.95 Da        Isoelectric Point: 7.7852

>NTDB_id=994158 AAG895_RS14990 WP_345792788.1 3302293..3303144(+) (pilD) [Thauera sp. JM12B12]
MRELLNDPAAFAALASLLGLFVGSFLNVVIHRLPQMMTRDWHAQAAELRGEEAPATERFNLATPRSRCPHCGHAIGALEN
IPVVSYVLLRGRCRHCGAGISKRYPIVEAFTAALSGYAAWHFGFGLAALGALLFIWAMVALAFIDLDTQLLPDDITLPLL
WLGLAFNLAGTYTELPAAVIGAMAGYLALWSVFWLFKLATGKEGMGYGDFKLLGAIGAWLGWQVLPLTILLSSLVGAVVG
IALIVFARHGRNVPIPFGPYLAAAGVLALFWGEALTTRYLGLL

Nucleotide


Download         Length: 852 bp        

>NTDB_id=994158 AAG895_RS14990 WP_345792788.1 3302293..3303144(+) (pilD) [Thauera sp. JM12B12]
ATGCGCGAGCTCCTGAACGATCCGGCGGCCTTCGCGGCCCTCGCCAGCCTGCTCGGCCTCTTCGTCGGCAGCTTCCTCAA
CGTCGTCATCCACCGCCTGCCGCAGATGATGACGCGCGACTGGCACGCCCAGGCCGCCGAGCTGCGCGGCGAGGAGGCGC
CGGCGACCGAGCGCTTCAACCTCGCCACCCCGCGCTCGCGCTGCCCCCACTGCGGCCACGCGATCGGCGCGCTCGAGAAC
ATCCCGGTGGTCAGCTACGTGCTGCTGCGCGGGCGCTGCCGTCATTGCGGCGCGGGCATCAGCAAGCGCTACCCGATCGT
CGAGGCCTTCACCGCAGCGTTGTCGGGCTATGCTGCCTGGCACTTCGGCTTCGGCCTCGCCGCGCTCGGCGCCCTGCTCT
TCATCTGGGCGATGGTGGCGCTGGCCTTCATCGACCTCGACACCCAGTTGCTGCCCGACGACATCACGCTGCCGCTGCTG
TGGCTGGGGCTGGCCTTCAACCTCGCCGGCACCTACACCGAGCTGCCGGCCGCGGTCATCGGCGCGATGGCCGGTTACCT
CGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTCGCCACCGGCAAGGAAGGCATGGGCTACGGCGACTTCAAGCTGCTCG
GCGCGATCGGGGCGTGGCTGGGGTGGCAGGTGTTGCCGCTGACCATCCTGCTGTCCTCGCTGGTCGGCGCTGTGGTCGGC
ATCGCCCTCATCGTCTTCGCACGCCACGGCCGCAACGTGCCGATTCCCTTCGGCCCCTATCTTGCGGCCGCCGGGGTGCT
CGCCCTGTTCTGGGGCGAGGCGCTGACCACGCGCTATCTCGGCCTGCTCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-122)

Type IV leader peptidase family (Peptidase_A24)

(133-241)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

53.818

97.173

0.523
  pilD Vibrio campbellii strain DS40M4

52.174

97.527

0.509
  pilD Acinetobacter baumannii D1279779

48.561

98.233

0.477
  pilD Acinetobacter nosocomialis M2

47.842

98.233

0.47
  pilD Neisseria gonorrhoeae MS11

49.416

90.813

0.449

Multiple sequence alignment