Detailed information NTG0994108

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AAH980_RS16660
• Genome accession: NZ_CP154856
• Coordinates: 3514176..3515294 (-)
• Length: 372 a.a.
• NCBI ID: WP_002052019.1
• Uniprot ID: A0A1V0YUM2
• Organism: Acinetobacter nosocomialis strain Ab25
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3509176..3520294

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAH980_RS16630 (AAH980_16630)	-	3510004..3510852 (+)	849	WP_009505210.1	class II glutamine amidotransferase	-
AAH980_RS16635 (AAH980_16635)	-	3511105..3511560 (+)	456	WP_000782976.1	bacteriohemerythrin	-
AAH980_RS16640 (AAH980_16640)	-	3511713..3512780 (+)	1068	WP_284080767.1	hypothetical protein	-
AAH980_RS16645 (AAH980_16645)	-	3512780..3513103 (+)	324	WP_002052062.1	RnfH family protein	-
AAH980_RS16650 (AAH980_16650)	bamE	3513159..3513557 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
AAH980_RS16655 (AAH980_16655)	fur	3513669..3514106 (+)	438	WP_002052029.1	ferric iron uptake transcriptional regulator	-
AAH980_RS16660 (AAH980_16660)	pilU	3514176..3515294 (-)	1119	WP_002052019.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AAH980_RS16665 (AAH980_16665)	pilT	3515322..3516359 (-)	1038	WP_006581387.1	type IV pilus twitching motility protein PilT	Machinery gene
AAH980_RS16670 (AAH980_16670)	-	3516486..3517178 (+)	693	WP_262750467.1	YggS family pyridoxal phosphate-dependent enzyme	-
AAH980_RS16675 (AAH980_16675)	-	3517286..3518128 (+)	843	WP_006581385.1	undecaprenyl-diphosphate phosphatase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41980.18 Da
• Isoelectric Point: 6.6944

>NTDB_id=994108 AAH980_RS16660 WP_002052019.1 3514176..3515294(-) (pilU) [Acinetobacter nosocomialis strain Ab25]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPTVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLANEGPDQITDEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=994108 AAH980_RS16660 WP_002052019.1 3514176..3515294(-) (pilU) [Acinetobacter nosocomialis strain Ab25]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCCGATCTGTTTATTACAGATGGTGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGCCTTTCAGGTGAAGTAATTGGTCAACTCTTACATTCCA
TCATGAGTGAAAAACAACGTAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATCATGAACCGCGATAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAACGTGACATGCCAGGTATGGTACTACGTCGAATTGAAACGAAAATTCCTTCAAT
TGATGACTTACAGTTGCCGACTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGTGCAACAG
GTACAGGTAAATCGACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAG
GACCCGATTGAGTTTATTCACGAACATGCGGGCTGCATTATTACTCAACGTGAAGTCGGTATTGATACGGACTCATTCGA
AATTGCGTTAAAAAACACGTTACGACAAGCGCCAGATGTGATCTTAATTGGTGAAATCCGCTCTCGTGAAGTCATGGACT
ACGCAATTGGCTTTGCTGAAACAGGACACCTTGTATTAGCTACAATGCATGCCAACAATGCCAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGACTTGTCTCTTAACTTAAAAGCCATGATTGCACA
GCAGCTTATTCCAACACCAGATGGCAATTCACGCCGTGCAGCGATTGAGATCTTAATTAACTCACCATTAATTTCAGACT
ATATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTGATGAAACGCTCACGTGAGCTTGGTATGCAGACCTTTGACCAA
GCTTTATTCGACTTATATAAAGCGGGCCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTAACGATTAAGCTTGCAAATGAAGGTCCTGATCAAATAACAGACGAAAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A1V0YUM2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.254	95.43	0.403