Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAEQ75_RS20275 Genome accession   NZ_CP154631
Coordinates   4299949..4301169 (-) Length   406 a.a.
NCBI ID   WP_179576719.1    Uniprot ID   -
Organism   Pseudomonas sediminis strain I4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4294949..4306169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAEQ75_RS20255 (AAEQ75_20255) - 4296118..4297017 (+) 900 WP_343350259.1 hydroxymethylglutaryl-CoA lyase -
  AAEQ75_RS20260 (AAEQ75_20260) - 4297322..4298149 (+) 828 WP_343350260.1 alpha/beta hydrolase -
  AAEQ75_RS20265 (AAEQ75_20265) - 4298136..4299089 (+) 954 WP_343350261.1 diguanylate cyclase -
  AAEQ75_RS20270 (AAEQ75_20270) - 4299167..4299907 (+) 741 WP_343350262.1 transporter substrate-binding domain-containing protein -
  AAEQ75_RS20275 (AAEQ75_20275) pilU 4299949..4301169 (-) 1221 WP_179576719.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAEQ75_RS20280 (AAEQ75_20280) - 4301318..4301596 (+) 279 WP_013715799.1 peptidylprolyl isomerase -
  AAEQ75_RS20285 (AAEQ75_20285) - 4301674..4302603 (-) 930 WP_343350264.1 LysR substrate-binding domain-containing protein -
  AAEQ75_RS20290 (AAEQ75_20290) - 4302743..4303138 (+) 396 WP_343350265.1 RidA family protein -
  AAEQ75_RS20295 (AAEQ75_20295) nfsB 4303181..4303831 (-) 651 WP_343350266.1 oxygen-insensitive NAD(P)H nitroreductase -
  AAEQ75_RS20300 (AAEQ75_20300) pdxY 4304217..4305095 (-) 879 WP_343350268.1 pyridoxal kinase PdxY -
  AAEQ75_RS20305 (AAEQ75_20305) - 4305278..4306033 (+) 756 WP_343350269.1 hypothetical protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44735.05 Da        Isoelectric Point: 6.3579

>NTDB_id=993869 AAEQ75_RS20275 WP_179576719.1 4299949..4301169(-) (pilU) [Pseudomonas sediminis strain I4]
MDLQAMLKILSTQDGSDLYLSTGAPPCAKFNGVLKALSQEPMKAGDVAAIADSIMDSAQREEFERELEMNLAISLAGVGR
FRINIFKQRNEVSIVARNIKLDIPRFEDLKLPEVLLSTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLKAFVSQRLVKTVDGKRRAAVEVMLGTPTVRDLIKRNEFSELKEIMEKSKNLGMQTFDQAL
IDLVHEGAIDEEEAVKNADSANNVRLKLKLHRDNPANARPAHAVAAAPAAQPVAAKSAAAGDWGLELKLEDIEQEQLPED
PGRQGI

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=993869 AAEQ75_RS20275 WP_179576719.1 4299949..4301169(-) (pilU) [Pseudomonas sediminis strain I4]
ATGGATCTCCAAGCAATGCTGAAAATCCTGTCCACTCAGGATGGTTCTGATCTCTATCTGTCCACCGGCGCGCCGCCCTG
CGCGAAGTTCAATGGCGTGCTCAAGGCGCTGAGCCAGGAGCCGATGAAGGCGGGCGATGTGGCGGCCATTGCCGATTCGA
TCATGGATAGTGCCCAGCGTGAGGAATTCGAGCGCGAACTGGAGATGAATCTGGCGATATCGCTGGCTGGCGTCGGGCGT
TTTCGCATCAACATCTTCAAACAGCGCAACGAGGTGTCCATCGTCGCGCGCAACATCAAGCTGGATATTCCTCGCTTCGA
AGACCTGAAACTGCCCGAGGTGCTGCTCAGCACCGTAATGGAAAAGCGCGGCCTGGTGCTGTTCGTTGGCGGCACCGGTT
CAGGCAAGTCGACCTCCCTGGCGGCGCTGATCGACTACCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAAGAC
CCGGTGGAGTACGTGCACCGGCACAAGAAATCGATCATCAACCAGCGTGAGGTGGGCGTGGACACCCGCAGCTTCCATGC
GGCATTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATTCTGATCGGTGAGATCCGCGACCGTGAAACCATGGAGCACG
CCCTGGCCTTTGCCGACACCGGCCATCTGGCGATCTCCACGTTGCATGCCAATAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGACCGCCGCCCGCAGTTGCTCAACGACCTGGGCAACAACCTCAAGGCGTTCGTCTCCCAGCG
CCTGGTCAAGACCGTCGATGGCAAGCGCCGCGCGGCGGTGGAGGTGATGCTCGGCACGCCGACGGTGCGCGACCTGATCA
AGCGCAACGAGTTTTCCGAACTGAAGGAAATCATGGAGAAGTCCAAGAACCTCGGCATGCAGACTTTCGACCAGGCGCTG
ATCGATCTGGTGCACGAAGGTGCAATCGACGAGGAAGAGGCGGTGAAGAACGCCGACTCGGCGAACAACGTGCGCCTGAA
ACTCAAGCTGCATCGCGACAACCCGGCCAACGCACGGCCTGCACATGCAGTAGCTGCTGCCCCTGCGGCGCAGCCCGTTG
CAGCGAAAAGTGCTGCAGCCGGTGATTGGGGGCTGGAACTGAAGCTGGAGGATATCGAGCAGGAGCAGTTGCCAGAAGAT
CCGGGGCGGCAGGGTATTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.41

86.453

0.488
  pilU Acinetobacter baylyi ADP1

51.989

86.7

0.451
  pilU Vibrio cholerae strain A1552

51.841

86.946

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.44

96.798

0.382

Multiple sequence alignment