Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAH969_RS02195 Genome accession   NZ_CP154349
Coordinates   469196..470344 (+) Length   382 a.a.
NCBI ID   WP_003100959.1    Uniprot ID   G3XCX3
Organism   Pseudomonas aeruginosa strain PAE3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 445345..496021 469196..470344 within 0


Gene organization within MGE regions


Location: 445345..496021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAH969_RS02060 (AAH969_02060) rsmD 445386..445982 (-) 597 WP_003102864.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  AAH969_RS02065 (AAH969_02065) - 445982..447469 (-) 1488 WP_003102862.1 pitrilysin family protein -
  AAH969_RS02070 (AAH969_02070) - 447462..448859 (-) 1398 WP_003084498.1 pitrilysin family protein -
  AAH969_RS02075 (AAH969_02075) ftsY 448980..450308 (+) 1329 WP_031757009.1 signal recognition particle-docking protein FtsY -
  AAH969_RS02080 (AAH969_02080) ftsE 450305..450976 (+) 672 WP_003084505.1 cell division ATP-binding protein FtsE -
  AAH969_RS02085 (AAH969_02085) ftsX 450976..451983 (+) 1008 WP_003084508.1 permease-like cell division protein FtsX -
  AAH969_RS02090 (AAH969_02090) rpoH 452097..452951 (+) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  AAH969_RS02095 (AAH969_02095) - 453016..453621 (+) 606 WP_003084511.1 hypothetical protein -
  AAH969_RS02100 (AAH969_02100) mtgA 453626..454357 (-) 732 WP_073658746.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  AAH969_RS02105 (AAH969_02105) - 454394..454771 (+) 378 WP_003084513.1 DUF423 domain-containing protein -
  AAH969_RS02110 (AAH969_02110) thiS 454874..455074 (+) 201 WP_003084514.1 sulfur carrier protein ThiS -
  AAH969_RS02115 (AAH969_02115) - 455133..455930 (+) 798 WP_003084517.1 thiazole synthase -
  AAH969_RS02120 (AAH969_02120) trmB 456084..456755 (+) 672 WP_003118800.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  AAH969_RS02125 (AAH969_02125) - 456918..458264 (+) 1347 WP_003114884.1 DUF1329 domain-containing protein -
  AAH969_RS02130 (AAH969_02130) - 458277..458534 (-) 258 WP_003084524.1 hypothetical protein -
  AAH969_RS02135 (AAH969_02135) - 458596..458919 (-) 324 WP_003084525.1 DUF3392 domain-containing protein -
  AAH969_RS02140 (AAH969_02140) hemW 459000..460154 (-) 1155 WP_003114885.1 radical SAM family heme chaperone HemW -
  AAH969_RS02145 (AAH969_02145) rdgB 460275..460868 (-) 594 WP_031757006.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  AAH969_RS02150 (AAH969_02150) - 460865..461284 (-) 420 WP_003100965.1 DUF4426 domain-containing protein -
  AAH969_RS02155 (AAH969_02155) metW 461416..462036 (-) 621 WP_003084530.1 methionine biosynthesis protein MetW -
  AAH969_RS02160 (AAH969_02160) - 462044..463183 (-) 1140 WP_003084535.1 homoserine O-acetyltransferase -
  AAH969_RS02165 (AAH969_02165) - 463272..465239 (-) 1968 WP_003121751.1 dynamin-like GTPase family protein -
  AAH969_RS02170 (AAH969_02170) - 465347..465643 (-) 297 WP_031628111.1 DUF167 domain-containing protein -
  AAH969_RS02175 (AAH969_02175) - 465640..466233 (-) 594 WP_003084544.1 YggT family protein -
  AAH969_RS02180 (AAH969_02180) proC 466244..467065 (-) 822 WP_031757004.1 pyrroline-5-carboxylate reductase -
  AAH969_RS02185 (AAH969_02185) - 467077..467769 (-) 693 WP_003084549.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAH969_RS02190 (AAH969_02190) pilT 467984..469018 (+) 1035 WP_003084552.1 type IV pilus twitching motility protein PilT Machinery gene
  AAH969_RS02195 (AAH969_02195) pilU 469196..470344 (+) 1149 WP_003100959.1 type IV pilus ATPase PilU Machinery gene
  AAH969_RS02200 (AAH969_02200) czcD 470352..471251 (-) 900 WP_003100957.1 CDF family zinc transporter CzcD -
  AAH969_RS02205 (AAH969_02205) - 471406..471810 (+) 405 WP_003084560.1 NINE protein -
  AAH969_RS02210 (AAH969_02210) - 472053..473426 (+) 1374 WP_003084562.1 pyridoxal-phosphate dependent enzyme -
  AAH969_RS02215 (AAH969_02215) - 473423..474607 (+) 1185 WP_003100953.1 cystathionine gamma-synthase -
  AAH969_RS02220 (AAH969_02220) - 474834..476105 (-) 1272 WP_003100951.1 dihydroorotase -
  AAH969_RS02225 (AAH969_02225) - 476102..477106 (-) 1005 WP_003084569.1 aspartate carbamoyltransferase catalytic subunit -
  AAH969_RS02230 (AAH969_02230) pyrR 477130..477642 (-) 513 WP_003084574.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  AAH969_RS02235 (AAH969_02235) ruvX 477754..478188 (-) 435 WP_003084575.1 Holliday junction resolvase RuvX -
  AAH969_RS02240 (AAH969_02240) - 478188..478757 (-) 570 WP_003084577.1 YqgE/AlgH family protein -
  AAH969_RS02245 (AAH969_02245) - 478806..479699 (-) 894 WP_003162015.1 energy transducer TonB -
  AAH969_RS02250 (AAH969_02250) gshB 479846..480799 (-) 954 WP_003100943.1 glutathione synthase -
  AAH969_RS02255 (AAH969_02255) pilG 481054..481461 (+) 408 WP_003084583.1 twitching motility response regulator PilG Regulator
  AAH969_RS02260 (AAH969_02260) pilH 481508..481873 (+) 366 WP_003084587.1 twitching motility response regulator PilH -
  AAH969_RS02265 (AAH969_02265) - 481924..482460 (+) 537 WP_003084590.1 chemotaxis protein CheW -
  AAH969_RS02270 (AAH969_02270) pilJ 482545..484593 (+) 2049 Protein_452 chemotaxis chemoreceptor PilJ -
  AAH969_RS02275 (AAH969_02275) pilK 484654..485529 (+) 876 WP_031757002.1 type 4 fimbrial methyltransferase PilK -
  AAH969_RS02280 (AAH969_02280) chpA 485541..492959 (+) 7419 Protein_454 chemotaxis signal transduction system protein ChpA -
  AAH969_RS02285 (AAH969_02285) - 492952..493983 (+) 1032 WP_003084598.1 chemotaxis protein CheB -
  AAH969_RS02290 (AAH969_02290) - 493980..494486 (+) 507 WP_003106119.1 chemotaxis protein CheW -
  AAH969_RS02295 (AAH969_02295) - 494494..495288 (+) 795 WP_003117898.1 AraC family transcriptional regulator -
  AAH969_RS02300 (AAH969_02300) - 495364..495975 (+) 612 WP_003084610.1 LysE family translocator -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 42532.81 Da        Isoelectric Point: 6.7792

>NTDB_id=992989 AAH969_RS02195 WP_003100959.1 469196..470344(+) (pilU) [Pseudomonas aeruginosa strain PAE3]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=992989 AAH969_RS02195 WP_003100959.1 469196..470344(+) (pilU) [Pseudomonas aeruginosa strain PAE3]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XCX3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

88.482

100

0.885
  pilU Acinetobacter baylyi ADP1

65.266

93.455

0.61
  pilU Vibrio cholerae strain A1552

60.286

91.623

0.552
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.068

88.743

0.382

Multiple sequence alignment