Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   MIDG2331_RS01020 Genome accession   NZ_LN908249
Coordinates   222474..223772 (+) Length   432 a.a.
NCBI ID   WP_005613635.1    Uniprot ID   A0A3G2M2V1
Organism   Actinobacillus pleuropneumoniae serovar 8 isolate MIDG2331     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 217474..228772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIDG2331_RS00995 (MIDG2331_00202) - 217646..220213 (-) 2568 WP_005595965.1 penicillin-binding protein 1A -
  MIDG2331_RS01000 (MIDG2331_00203) - 220352..221032 (+) 681 WP_039708937.1 hypothetical protein -
  MIDG2331_RS01005 (MIDG2331_00204) - 221014..221535 (+) 522 WP_005595970.1 hypothetical protein -
  MIDG2331_RS01010 (MIDG2331_00205) - 221634..222065 (+) 432 WP_005595972.1 hypothetical protein -
  MIDG2331_RS01015 (MIDG2331_00206) - 222058..222462 (+) 405 WP_005595974.1 hypothetical protein -
  MIDG2331_RS01020 (MIDG2331_00207) comE 222474..223772 (+) 1299 WP_005613635.1 type IV pilus secretin PilQ Machinery gene
  MIDG2331_RS01025 (MIDG2331_00208) nusB 223898..224311 (+) 414 WP_005595979.1 transcription antitermination factor NusB -
  MIDG2331_RS01030 (MIDG2331_00209) thiL 224376..225341 (+) 966 WP_005618774.1 thiamine-phosphate kinase -
  MIDG2331_RS01035 (MIDG2331_00210) - 225354..225827 (+) 474 WP_005603437.1 phosphatidylglycerophosphatase A -
  MIDG2331_RS01040 (MIDG2331_00211) - 225840..226466 (+) 627 WP_039709529.1 LysE family transporter -
  MIDG2331_RS01045 (MIDG2331_00212) - 226514..227551 (-) 1038 WP_058230448.1 TrmH family RNA methyltransferase -
  MIDG2331_RS01050 (MIDG2331_00213) suhB 227717..228529 (+) 813 WP_005595989.1 inositol-1-monophosphatase -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 47775.91 Da        Isoelectric Point: 9.4227

>NTDB_id=992552 MIDG2331_RS01020 WP_005613635.1 222474..223772(+) (comE) [Actinobacillus pleuropneumoniae serovar 8 isolate MIDG2331]
MRKMIWLLLFILTPVMANTFSLSLKNAPTAEILSYLAEEYGKNIVLSDNIETNTTLRIENSDFDSVLKSITRANKLTSAY
EKQIYFIGHKKDEKAATAIGVNSDLLKPKLITKTIKLDYAKAAEVIESLTKGSGHFLSENGYLHFDDRSNSLIIKDSPES
MKNIVQLIRNLDKPTEQIAIEARIVTISSENLQELGVRWGIFAPTNGHHKIAGSLEANGLPNTNRLNVNFPVNNAASIAL
QVAKINGRVLDLELTALEQENNVEIIASPRLLTTNKKPASIKQGTEVPYVLYNRKDEVKNIEFKEAVLGLQVTPHISNDK
QILLDLVVTQNSPNSTGSTLQGLVTIDKQELNTQVFAQHGETIVLGGIFQHLTAKGEDRVPILGSIPVLKKLFSHSSDKI
SKRELVIFVTPYIVKNGQPQISSHSSQKLAPK

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=992552 MIDG2331_RS01020 WP_005613635.1 222474..223772(+) (comE) [Actinobacillus pleuropneumoniae serovar 8 isolate MIDG2331]
ATGCGAAAAATGATTTGGTTGCTCCTGTTTATATTAACGCCGGTTATGGCAAATACTTTTTCTCTCTCGCTGAAAAATGC
GCCGACTGCGGAAATTTTAAGTTATTTAGCCGAAGAATACGGTAAAAATATCGTGTTAAGCGACAATATTGAGACAAATA
CTACATTAAGAATTGAAAATAGTGATTTTGACAGCGTATTAAAAAGTATTACTCGGGCGAATAAACTCACGAGTGCATAC
GAAAAACAGATCTATTTTATCGGGCATAAAAAAGATGAGAAAGCGGCTACGGCAATAGGCGTAAATTCTGATTTATTGAA
ACCTAAATTGATCACTAAGACAATAAAATTAGATTATGCGAAGGCGGCGGAAGTGATTGAATCTTTAACTAAAGGAAGCG
GCCATTTTTTATCGGAAAACGGCTATTTGCATTTTGATGATCGTAGTAACAGTTTGATTATTAAAGACAGTCCGGAATCG
ATGAAAAATATCGTGCAATTAATCAGAAACTTAGATAAACCGACCGAACAGATTGCAATTGAAGCCAGAATTGTCACGAT
AAGCAGTGAGAATTTACAAGAATTAGGTGTGCGTTGGGGGATATTTGCTCCTACAAACGGACACCATAAAATTGCCGGTT
CGCTTGAAGCGAACGGATTACCGAATACTAATCGTTTAAACGTAAATTTTCCGGTAAATAATGCCGCTTCTATTGCACTA
CAAGTGGCGAAAATTAATGGGCGAGTGCTTGATTTGGAACTTACCGCTTTGGAACAAGAAAATAATGTTGAGATTATCGC
CAGCCCTCGTTTATTAACCACCAATAAGAAGCCGGCAAGCATTAAGCAAGGAACGGAAGTTCCTTATGTGCTGTATAACC
GTAAAGACGAAGTGAAAAATATCGAATTTAAAGAAGCGGTTTTAGGTTTACAAGTGACGCCGCATATTTCAAACGATAAG
CAAATTTTGCTCGATTTGGTAGTCACCCAAAACTCACCTAATTCGACCGGTTCAACCCTACAAGGCTTAGTGACGATTGA
TAAGCAGGAACTGAATACGCAAGTATTTGCTCAGCATGGCGAAACGATTGTGCTAGGCGGTATTTTTCAGCATTTAACCG
CAAAAGGTGAGGACAGAGTGCCGATTTTAGGTTCGATTCCGGTACTTAAAAAGTTATTTAGCCATTCTAGTGATAAAATT
AGTAAGCGTGAACTAGTGATTTTTGTGACGCCTTACATTGTTAAAAACGGACAACCGCAAATTTCCTCACATTCTTCACA
GAAATTAGCGCCAAAATAG

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(112-177)

Bacterial type II and III secretion system protein (Secretin)

(256-415)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G2M2V1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

66.506

96.065

0.639
  comE Haemophilus influenzae Rd KW20

53.037

99.074

0.525
  comE Haemophilus influenzae 86-028NP

52.804

99.074

0.523
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.41

96.065

0.407
  pilQ Vibrio cholerae strain A1552

42.41

96.065

0.407
  pilQ Vibrio campbellii strain DS40M4

40.326

99.306

0.4