Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LA521A_RS15920 Genome accession   NZ_AP027041
Coordinates   3510458..3511516 (-) Length   352 a.a.
NCBI ID   WP_281779829.1    Uniprot ID   -
Organism   Lysobacter auxotrophicus strain 5-21a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3505458..3516516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA521A_RS15900 (LA521A_31340) - 3506493..3508430 (-) 1938 WP_281779825.1 type IV pilus secretin PilQ family protein -
  LA521A_RS15905 (LA521A_31350) - 3508449..3509000 (-) 552 WP_281779826.1 pilus assembly protein PilP -
  LA521A_RS15910 (LA521A_31360) pilO 3509002..3509676 (-) 675 WP_281779827.1 type 4a pilus biogenesis protein PilO -
  LA521A_RS15915 (LA521A_31370) - 3509673..3510458 (-) 786 WP_281779828.1 PilN domain-containing protein -
  LA521A_RS15920 (LA521A_31380) pilM 3510458..3511516 (-) 1059 WP_281779829.1 pilus assembly protein PilM Machinery gene
  LA521A_RS15925 (LA521A_31390) - 3511798..3514290 (+) 2493 WP_281779830.1 penicillin-binding protein 1A -
  LA521A_RS15930 (LA521A_31400) - 3514505..3515158 (+) 654 WP_281779831.1 hypothetical protein -
  LA521A_RS15935 (LA521A_31410) - 3515165..3515779 (-) 615 WP_281779832.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37578.96 Da        Isoelectric Point: 4.4991

>NTDB_id=99238 LA521A_RS15920 WP_281779829.1 3510458..3511516(-) (pilM) [Lysobacter auxotrophicus strain 5-21a]
MGIVTKSQPAVVGVDISSTAVKLLQLSRAGNRYRVEHYAVEPLPPNAVVEKSIVEVEAVGEAIRRAVARSGTRAKHAAAA
VAGSSVITKVIPMPGDLDEDELESQVELEAVNYVPYPIEEVNHDFEVLGPMPGSPDMLQVLLAASRSENVEVRASALEIG
GLTPKVMDVEAFALENAFALVADQLGGPRDGLIALVDSGATMTTLNVLRNGRSLYAREQVFGGKQLTDEVMRRYGLSYEE
AGLAKRQGGLPESYEAEVLEPFKEAMVQQISRLLQFFYAGSEFNRVDQIVLAGGCASIAGIAEMVEEQLGVPTTIANPLA
HMTLGPRVQAHALAQDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=99238 LA521A_RS15920 WP_281779829.1 3510458..3511516(-) (pilM) [Lysobacter auxotrophicus strain 5-21a]
GTGGGGATCGTCACAAAAAGCCAGCCGGCGGTCGTCGGCGTGGACATCAGTTCGACTGCCGTAAAGCTTTTGCAGCTCTC
GCGGGCCGGCAATCGCTATCGCGTCGAGCATTACGCAGTCGAACCGCTGCCGCCGAACGCCGTCGTCGAGAAGAGCATCG
TCGAGGTCGAGGCCGTGGGCGAAGCGATCCGTCGCGCCGTCGCGCGCTCGGGCACTCGCGCCAAGCACGCCGCGGCCGCG
GTCGCCGGTTCGTCGGTCATCACCAAGGTCATCCCGATGCCGGGCGACCTGGACGAGGACGAGCTCGAGTCGCAGGTCGA
ACTGGAGGCGGTCAACTACGTCCCGTACCCGATCGAAGAGGTCAACCACGATTTCGAAGTGCTGGGCCCGATGCCCGGCA
GCCCGGACATGCTGCAGGTGCTGCTCGCCGCGTCGCGTTCGGAAAACGTCGAAGTGCGCGCCTCGGCGCTGGAGATCGGC
GGCCTCACCCCGAAGGTGATGGACGTTGAAGCCTTCGCGCTGGAGAACGCCTTCGCGCTGGTCGCCGACCAGCTGGGCGG
TCCGCGCGACGGCCTGATCGCGCTGGTCGATTCGGGCGCGACGATGACCACGCTCAACGTGCTGCGCAACGGCCGCAGCC
TGTATGCACGCGAGCAGGTCTTCGGCGGCAAGCAGCTCACCGACGAGGTGATGCGCCGCTACGGCCTGAGCTACGAGGAA
GCCGGCCTGGCCAAGCGCCAGGGCGGGCTGCCGGAGAGCTACGAAGCCGAAGTGCTCGAACCTTTCAAGGAGGCGATGGT
CCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTACGCGGGCAGCGAGTTCAACCGCGTCGACCAGATCGTGCTCGCCGGTG
GCTGCGCGTCCATCGCCGGCATTGCCGAGATGGTCGAGGAACAGCTCGGCGTGCCGACCACCATCGCCAACCCGCTCGCC
CACATGACGCTCGGGCCGCGCGTGCAGGCGCATGCGCTGGCCCAGGACGCCCCGGCCCTGATGATCGCCTGCGGCCTGGC
CCTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.003

99.148

0.506
  comM Acinetobacter nosocomialis M2

50.716

99.148

0.503
  comM Acinetobacter baylyi ADP1

49.568

98.58

0.489
  pilM Legionella pneumophila strain ERS1305867

45.748

96.875

0.443

Multiple sequence alignment