Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACGHAS_RS02045 Genome accession   NZ_AP035801
Coordinates   438679..439356 (-) Length   225 a.a.
NCBI ID   WP_000178174.1    Uniprot ID   A0A0H2VBG7
Organism   Escherichia coli strain E. coli JCM:5491     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 433679..444356
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHAS_RS02030 (ReJCM5491_03950) chiA 435037..437739 (+) 2703 WP_000773147.1 bifunctional chitinase/lysozyme -
  ACGHAS_RS02035 (ReJCM5491_03960) bfd 437908..438102 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  ACGHAS_RS02040 (ReJCM5491_03970) bfr 438174..438650 (+) 477 WP_000675504.1 bacterioferritin -
  ACGHAS_RS02045 (ReJCM5491_03980) pilD 438679..439356 (-) 678 WP_000178174.1 prepilin peptidase GspO Machinery gene
  ACGHAS_RS02050 (ReJCM5491_03990) gspM 439356..439817 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  ACGHAS_RS02055 (ReJCM5491_04000) gspL 439814..440977 (-) 1164 WP_001304915.1 type II secretion system protein GspL -
  ACGHAS_RS02060 (ReJCM5491_04010) gspK 440992..441975 (-) 984 Protein_409 type II secretion system minor pseudopilin GspK -
  ACGHAS_RS02065 (ReJCM5491_04020) gspJ 441968..442555 (-) 588 WP_000610637.1 type II secretion system minor pseudopilin GspJ -
  ACGHAS_RS02070 (ReJCM5491_04030) gspI 442548..442925 (-) 378 WP_001041742.1 type II secretion system minor pseudopilin GspI -
  ACGHAS_RS02075 (ReJCM5491_04040) gspH 442922..443431 (-) 510 WP_001076033.1 type II secretion system minor pseudopilin GspH -
  ACGHAS_RS02080 (ReJCM5491_04050) gspG 443439..443876 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24866.76 Da        Isoelectric Point: 7.6364

>NTDB_id=99091 ACGHAS_RS02045 WP_000178174.1 438679..439356(-) (pilD) [Escherichia coli strain E. coli JCM:5491]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTIPTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=99091 ACGHAS_RS02045 WP_000178174.1 438679..439356(-) (pilD) [Escherichia coli strain E. coli JCM:5491]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTACCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATACCCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VBG7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387

Multiple sequence alignment