Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AAGT77_RS12680 Genome accession   NZ_CP152380
Coordinates   2729962..2730837 (+) Length   291 a.a.
NCBI ID   WP_342630809.1    Uniprot ID   -
Organism   Marinobacter alkaliphilus strain SBY-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2724962..2735837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGT77_RS12665 (AAGT77_12665) - 2726078..2726701 (-) 624 WP_342630806.1 pilin -
  AAGT77_RS12670 (AAGT77_12670) pilB 2726954..2728666 (+) 1713 WP_342630807.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAGT77_RS12675 (AAGT77_12675) pilC 2728669..2729889 (+) 1221 WP_342630808.1 type II secretion system F family protein Machinery gene
  AAGT77_RS12680 (AAGT77_12680) pilD 2729962..2730837 (+) 876 WP_342630809.1 A24 family peptidase Machinery gene
  AAGT77_RS12685 (AAGT77_12685) coaE 2730934..2731539 (+) 606 WP_342630810.1 dephospho-CoA kinase -
  AAGT77_RS12690 (AAGT77_12690) - 2731622..2732620 (+) 999 WP_342630811.1 hypothetical protein -
  AAGT77_RS12695 (AAGT77_12695) tsaA 2732622..2733371 (+) 750 WP_342630812.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  AAGT77_RS12700 (AAGT77_12700) - 2733415..2735769 (+) 2355 WP_342630813.1 response regulator -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32122.23 Da        Isoelectric Point: 6.6786

>NTDB_id=988774 AAGT77_RS12680 WP_342630809.1 2729962..2730837(+) (pilD) [Marinobacter alkaliphilus strain SBY-1]
MPSLDQFLSTPWLLYLTVTLFSLCIGSFLNVVILRLPKMMQQEWRCQCEEFLELPEKQRKQETPLSLSKPASTCPSCGHQ
IRAWENIPVISWLVLRGKCSSCGTSISPRYPIIEAITAIFSVVTVALLGPTEAALWALLLVWALVALTMIDFDTQLLPDS
ITLPLMWLGLVLNYFGVLTDFNSAFWGAVAGYLSLWSVYWLFKLVTGKEGMGHGDFKLLAALGAWLGWQLLPAVILLSSL
VGAVVGIALMVFKKHGREVPIPFGPYLAAAGLLGLWFGAEIQAIWFGFLGV

Nucleotide


Download         Length: 876 bp        

>NTDB_id=988774 AAGT77_RS12680 WP_342630809.1 2729962..2730837(+) (pilD) [Marinobacter alkaliphilus strain SBY-1]
ATGCCCTCTCTAGACCAATTCCTCTCCACCCCCTGGCTCCTATACCTAACCGTCACCCTATTCTCCCTCTGCATCGGCAG
CTTCCTGAACGTCGTCATCTTACGCCTGCCAAAAATGATGCAGCAGGAATGGCGTTGCCAGTGTGAAGAGTTTCTGGAGT
TACCGGAAAAGCAGCGCAAGCAGGAAACACCCCTTTCCCTCTCCAAGCCAGCCTCCACCTGCCCATCCTGCGGTCATCAG
ATTCGGGCCTGGGAGAATATTCCAGTGATCAGTTGGCTAGTACTGCGGGGCAAGTGCTCCTCTTGTGGAACGTCGATTTC
ACCGCGCTACCCGATTATTGAAGCCATCACCGCGATCTTCTCAGTTGTGACCGTCGCTTTACTGGGGCCGACTGAAGCAG
CGCTCTGGGCCCTGCTGCTGGTATGGGCCCTGGTGGCGCTGACGATGATCGATTTCGACACGCAGTTGCTACCAGACAGC
ATTACCCTGCCGCTAATGTGGCTGGGGCTGGTGTTGAATTATTTTGGCGTGCTGACGGACTTTAACAGTGCCTTCTGGGG
TGCAGTGGCAGGGTATTTGTCGCTGTGGTCGGTGTACTGGCTATTCAAGCTGGTGACTGGCAAGGAAGGCATGGGGCACG
GGGATTTCAAGCTGTTGGCGGCGCTGGGTGCCTGGTTGGGGTGGCAGTTGTTGCCGGCGGTGATTTTGCTGTCGTCCCTG
GTGGGGGCGGTGGTTGGTATTGCGCTGATGGTGTTCAAAAAGCACGGGCGTGAGGTGCCGATTCCGTTTGGGCCTTATCT
GGCGGCAGCTGGGTTGTTGGGGCTTTGGTTTGGGGCGGAGATTCAGGCGATTTGGTTTGGGTTTTTGGGGGTTTAG

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-125)

Type IV leader peptidase family (Peptidase_A24)

(138-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

54.804

96.564

0.529
  pilD Acinetobacter nosocomialis M2

54.448

96.564

0.526
  pilD Vibrio campbellii strain DS40M4

51.761

97.595

0.505
  pilD Vibrio cholerae strain A1552

52.143

96.22

0.502
  pilD Neisseria gonorrhoeae MS11

50.397

86.598

0.436

Multiple sequence alignment