Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   AAG562_RS00055 Genome accession   NZ_CP152340
Coordinates   11863..12927 (-) Length   354 a.a.
NCBI ID   WP_003283479.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain NCIMB_885     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 10373..11401 11863..12927 flank 462


Gene organization within MGE regions


Location: 10373..12927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG562_RS00045 (AAG562_00045) - 10373..11401 (-) 1029 WP_003283602.1 IS30-like element ISPsp7 family transposase -
  AAG562_RS00050 (AAG562_00050) - 11456..11863 (-) 408 Protein_9 PilN domain-containing protein -
  AAG562_RS00055 (AAG562_00055) comM 11863..12927 (-) 1065 WP_003283479.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37723.26 Da        Isoelectric Point: 4.6346

>NTDB_id=988427 AAG562_RS00055 WP_003283479.1 11863..12927(-) (comM) [Stutzerimonas stutzeri strain NCIMB_885]
MLGLLTKKANTLLGIDISSTSVKLLELSRSGSRYRVEAYAVEPLPPNAVVEKNIAELEGVGQALQRVVAKAKTGCKSAVV
AVSGSAVITKTIEMDAGLSDEELENQLKIEADQYIPYPLEEVAIDFEVQGPAPRAPGRVEVLLAACRKENVEIREAALAL
AGLTAKIVDVEAYALERSFGLLAPQLGAGHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSQ
EEAGLAKKQGGLPDDYDSEVLLPFKEAVVQQVSRSLQFFFAAGQFHDVDCILLAGGTASIPGLDHMIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=988427 AAG562_RS00055 WP_003283479.1 11863..12927(-) (comM) [Stutzerimonas stutzeri strain NCIMB_885]
GTGCTAGGGCTCCTCACTAAGAAAGCGAACACGCTGCTTGGGATCGATATCAGTTCGACCTCAGTCAAGCTCCTCGAATT
AAGTCGATCCGGTAGTCGGTACCGTGTCGAGGCTTATGCTGTCGAGCCGCTTCCGCCGAACGCCGTTGTCGAAAAGAATA
TTGCAGAGCTCGAAGGGGTCGGGCAGGCATTACAGCGAGTTGTTGCCAAGGCTAAGACGGGCTGCAAGTCAGCCGTCGTT
GCAGTATCTGGCTCCGCGGTCATTACCAAGACCATCGAGATGGATGCTGGATTGTCCGATGAAGAACTCGAAAACCAGCT
GAAGATCGAGGCGGATCAGTACATTCCTTACCCGCTGGAAGAAGTTGCAATCGACTTCGAGGTTCAGGGGCCGGCCCCTC
GCGCTCCGGGCCGGGTCGAAGTCCTGCTTGCTGCTTGCCGCAAGGAAAACGTTGAAATTCGCGAAGCTGCTTTGGCTTTG
GCCGGGCTGACTGCAAAGATAGTCGATGTCGAAGCCTATGCCCTTGAGCGCTCTTTCGGCCTGCTCGCCCCGCAATTGGG
AGCAGGGCACGACGAGCTGACCGTGGCGGTTGTTGATATAGGCGCGACCATGACCACGCTTAGCGTTCTGCATAACGGTC
GTACCATCTACACCCGTGAGCAGTTGTTCGGCGGTCGCCAGCTGACCGAAGAAATCCAGCGGCGTTACGGCCTCTCCCAA
GAGGAAGCCGGCCTTGCCAAGAAACAAGGTGGGCTCCCGGATGACTACGACAGTGAGGTTCTGCTTCCGTTCAAAGAAGC
GGTTGTCCAGCAGGTTTCGCGCTCCTTGCAGTTCTTCTTCGCCGCCGGGCAGTTTCACGATGTCGATTGCATTCTGCTTG
CGGGCGGAACTGCCTCCATCCCCGGACTTGATCACATGATCCAGCAAAAGATCGGCACTCAAACTCTAGTTGCGAACCCG
TTTGCCGATATGGCTTTGAGTAGCAAGGTCAACGCTGGTGCGCTGGCCAGCGATGCGCCCGCATTAATGATTGCGTGCGG
ATTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

52.825

100

0.528
  pilM Legionella pneumophila strain ERS1305867

46.328

100

0.463

Multiple sequence alignment