Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGC   Type   Machinery gene
Locus tag   MHB67_RS11350 Genome accession   NZ_CP152051
Coordinates   2256405..2256698 (-) Length   97 a.a.
NCBI ID   WP_024422977.1    Uniprot ID   A0A0M2EIN6
Organism   Bacillus sp. FSL H8-0516     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2251405..2261698
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHB67_RS11300 (MHB67_11300) sinR 2251572..2251907 (+) 336 WP_024422969.1 transcriptional regulator SinR Regulator
  MHB67_RS11305 (MHB67_11305) - 2251975..2252775 (-) 801 WP_024422970.1 TasA family protein -
  MHB67_RS11310 (MHB67_11310) - 2252830..2253402 (-) 573 WP_024422971.1 signal peptidase I -
  MHB67_RS11315 (MHB67_11315) tapA 2253455..2253982 (-) 528 WP_103131952.1 amyloid fiber anchoring/assembly protein TapA -
  MHB67_RS11320 (MHB67_11320) - 2254282..2254560 (+) 279 WP_171464609.1 DUF3889 domain-containing protein -
  MHB67_RS11325 (MHB67_11325) - 2254595..2254789 (-) 195 WP_024422974.1 YqzE family protein -
  MHB67_RS11330 (MHB67_11330) comGG 2254849..2255232 (-) 384 WP_103131951.1 competence type IV pilus minor pilin ComGG -
  MHB67_RS11335 (MHB67_11335) comGF 2255229..2255756 (-) 528 WP_258011119.1 competence type IV pilus minor pilin ComGF -
  MHB67_RS11340 (MHB67_11340) comGE 2255668..2255982 (-) 315 WP_073206282.1 competence type IV pilus minor pilin ComGE -
  MHB67_RS11345 (MHB67_11345) comGD 2255966..2256412 (-) 447 WP_103131949.1 competence type IV pilus minor pilin ComGD -
  MHB67_RS11350 (MHB67_11350) comGC 2256405..2256698 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  MHB67_RS11355 (MHB67_11355) comGB 2256715..2257755 (-) 1041 WP_103131948.1 competence type IV pilus assembly protein ComGB -
  MHB67_RS11360 (MHB67_11360) comGA 2257736..2258806 (-) 1071 WP_103131947.1 competence type IV pilus ATPase ComGA Machinery gene
  MHB67_RS11365 (MHB67_11365) - 2258984..2259859 (-) 876 WP_024422979.1 STAS domain-containing protein -
  MHB67_RS11375 (MHB67_11375) - 2260083..2260463 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHB67_RS11380 (MHB67_11380) - 2260676..2260918 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  MHB67_RS11385 (MHB67_11385) - 2260966..2261604 (-) 639 WP_024422981.1 MBL fold metallo-hydrolase -

Sequence


Protein


Download         Length: 97 a.a.        Molecular weight: 10686.62 Da        Isoelectric Point: 7.3218

>NTDB_id=986929 MHB67_RS11350 WP_024422977.1 2256405..2256698(-) (comGC) [Bacillus sp. FSL H8-0516]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQAIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIVIQNGNVKHE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=986929 MHB67_RS11350 WP_024422977.1 2256405..2256698(-) (comGC) [Bacillus sp. FSL H8-0516]
ATGAATGATAAAGGATTTACCCTGTTAGAAATGTTAATAGTTATGCTCGTAATATCCATTCTTTTACTCATTACCATACC
GAATATCACACGTCATCACCAAGCGATTCAGGCAAAGGGCTGTGAAGGGCTTAAATCAATGGTCAGCACACAAGTGATGG
CATATGAATTGGAGCATGATGGGAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGTTATGTCAAAAAGGGGTTAACT
TGTCCTAATGGAAAAGCGATTGTCATTCAAAACGGCAACGTGAAGCATGAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0M2EIN6

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67

  comGC Staphylococcus aureus MW2

42.222

92.784

0.392

  comGC Staphylococcus aureus N315

42.222

92.784

0.392


Multiple sequence alignment