Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MHB67_RS11360 Genome accession   NZ_CP152051
Coordinates   2257736..2258806 (-) Length   356 a.a.
NCBI ID   WP_103131947.1    Uniprot ID   -
Organism   Bacillus sp. FSL H8-0516     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2252736..2263806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHB67_RS11310 (MHB67_11310) - 2252830..2253402 (-) 573 WP_024422971.1 signal peptidase I -
  MHB67_RS11315 (MHB67_11315) tapA 2253455..2253982 (-) 528 WP_103131952.1 amyloid fiber anchoring/assembly protein TapA -
  MHB67_RS11320 (MHB67_11320) - 2254282..2254560 (+) 279 WP_171464609.1 DUF3889 domain-containing protein -
  MHB67_RS11325 (MHB67_11325) - 2254595..2254789 (-) 195 WP_024422974.1 YqzE family protein -
  MHB67_RS11330 (MHB67_11330) comGG 2254849..2255232 (-) 384 WP_103131951.1 competence type IV pilus minor pilin ComGG -
  MHB67_RS11335 (MHB67_11335) comGF 2255229..2255756 (-) 528 WP_258011119.1 competence type IV pilus minor pilin ComGF -
  MHB67_RS11340 (MHB67_11340) comGE 2255668..2255982 (-) 315 WP_073206282.1 competence type IV pilus minor pilin ComGE -
  MHB67_RS11345 (MHB67_11345) comGD 2255966..2256412 (-) 447 WP_103131949.1 competence type IV pilus minor pilin ComGD -
  MHB67_RS11350 (MHB67_11350) comGC 2256405..2256698 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  MHB67_RS11355 (MHB67_11355) comGB 2256715..2257755 (-) 1041 WP_103131948.1 competence type IV pilus assembly protein ComGB -
  MHB67_RS11360 (MHB67_11360) comGA 2257736..2258806 (-) 1071 WP_103131947.1 competence type IV pilus ATPase ComGA Machinery gene
  MHB67_RS11365 (MHB67_11365) - 2258984..2259859 (-) 876 WP_024422979.1 STAS domain-containing protein -
  MHB67_RS11375 (MHB67_11375) - 2260083..2260463 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHB67_RS11380 (MHB67_11380) - 2260676..2260918 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  MHB67_RS11385 (MHB67_11385) - 2260966..2261604 (-) 639 WP_024422981.1 MBL fold metallo-hydrolase -
  MHB67_RS11390 (MHB67_11390) - 2261762..2261935 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  MHB67_RS11395 (MHB67_11395) - 2261961..2262275 (-) 315 WP_024427984.1 MTH1187 family thiamine-binding protein -
  MHB67_RS11400 (MHB67_11400) - 2262352..2263605 (-) 1254 WP_133744200.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40442.95 Da        Isoelectric Point: 9.5360

>NTDB_id=986930 MHB67_RS11360 WP_103131947.1 2257736..2258806(-) (comGA) [Bacillus sp. FSL H8-0516]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRMIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRKGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDVIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGERCQLYCKLNRPIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=986930 MHB67_RS11360 WP_103131947.1 2257736..2258806(-) (comGA) [Bacillus sp. FSL H8-0516]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCATTTCGTGTTGATTCAGATTTAATCCAGCAGCGAATGATTGATAAAAAAAGCGGAG
AGCGGCTTATTGCACATTTTAAGTTTTTATCTTCTATGGATATCGGTGAAAAAAGGAGGCCGCAAAATGGCTCACTAGCT
GTGATGCTAAGAAAAGGTCAAGTATTTATTCGGATGTCTACTTTGCCGACAGTAAATGATGAGAGCTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGATTATTATCCTTTTTAA
ACCACTCGCATGGACTCATTTTATTTACTGGGCCAACAAATTCAGGGAAAACGACAACACTATATTCATTGATCCAGTTT
GCAAAAAAGAATTTTAACCGAAATATTATTACCCTTGAAGATCCTGTCGAAACAAGGAATGAAGAAGTGCTGCAGGTTCA
AGTGAATGAAAAAGCCGGCATCACATATGCCGCAGGATTACGCGCTATTTTAAGACATGATCCAGATGTGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCTAGAACGGCGATTAGAGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAACGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTTGAGCAAACGATGGTTGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAAGATGTCAGCTTTACTGTAAATTAAATCGAC
CGATCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTCGGCTATTTATCAAAAAACACTTACCA
TCGCTGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.689

99.438

0.643

Multiple sequence alignment