Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   MHH29_RS00250 Genome accession   NZ_CP152048
Coordinates   39702..39995 (-) Length   97 a.a.
NCBI ID   WP_008342186.1    Uniprot ID   A0A5K1NAM4
Organism   Bacillus sp. FSL K6-0046     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 34702..44995
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHH29_RS00200 (MHH29_00200) sinR 34866..35201 (+) 336 WP_017357978.1 helix-turn-helix domain-containing protein Regulator
  MHH29_RS00205 (MHH29_00205) - 35269..36069 (-) 801 WP_008342195.1 TasA family protein -
  MHH29_RS00210 (MHH29_00210) - 36128..36700 (-) 573 WP_017357979.1 signal peptidase I -
  MHH29_RS00215 (MHH29_00215) tapA 36750..37277 (-) 528 WP_056705241.1 amyloid fiber anchoring/assembly protein TapA -
  MHH29_RS00220 (MHH29_00220) - 37530..37856 (+) 327 WP_058336653.1 DUF3889 domain-containing protein -
  MHH29_RS00225 (MHH29_00225) - 37891..38085 (-) 195 WP_024718846.1 YqzE family protein -
  MHH29_RS00230 (MHH29_00230) comGG 38146..38529 (-) 384 WP_342494816.1 competence type IV pilus minor pilin ComGG -
  MHH29_RS00235 (MHH29_00235) comGF 38526..39023 (-) 498 WP_216894677.1 competence type IV pilus minor pilin ComGF -
  MHH29_RS00240 (MHH29_00240) comGE 38965..39279 (-) 315 WP_039166034.1 competence type IV pilus minor pilin ComGE -
  MHH29_RS00245 (MHH29_00245) comGD 39263..39709 (-) 447 WP_096881621.1 competence type IV pilus minor pilin ComGD -
  MHH29_RS00250 (MHH29_00250) comGC 39702..39995 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  MHH29_RS00255 (MHH29_00255) comGB 40012..41052 (-) 1041 WP_024718840.1 competence type IV pilus assembly protein ComGB -
  MHH29_RS00260 (MHH29_00260) comGA 41033..42103 (-) 1071 WP_024718839.1 competence type IV pilus ATPase ComGA Machinery gene
  MHH29_RS00265 (MHH29_00265) - 42561..43433 (-) 873 WP_035390819.1 STAS domain-containing protein -
  MHH29_RS00275 (MHH29_00275) - 43659..44039 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHH29_RS00280 (MHH29_00280) - 44252..44494 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10728.62 Da        Isoelectric Point: 6.7081

>NTDB_id=986767 MHH29_RS00250 WP_008342186.1 39702..39995(-) (comGC) [Bacillus sp. FSL K6-0046]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQSIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIFIQNGDVKNE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=986767 MHH29_RS00250 WP_008342186.1 39702..39995(-) (comGC) [Bacillus sp. FSL K6-0046]
ATGAATGATAAAGGATTTACACTGTTAGAAATGTTAATTGTCATGCTCGTGATCTCCATTCTTTTATTGATCACCATTCC
AAATATCACTCGGCATCATCAAAGCATTCAAGCAAAGGGCTGTGAAGGGCTGAAATCAATGGTGAGTACACAGGTAATGG
CATATGAATTAGAGCATGACGGCAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGCTATGTCAAAAAAGGATTAACT
TGTCCAAATGGAAAGGCAATTTTCATTCAAAACGGGGATGTGAAAAATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5K1NAM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67
  comGC Staphylococcus aureus MW2

41.304

94.845

0.392
  comGC Staphylococcus aureus N315

41.304

94.845

0.392

Multiple sequence alignment