Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MHH29_RS00260 Genome accession   NZ_CP152048
Coordinates   41033..42103 (-) Length   356 a.a.
NCBI ID   WP_024718839.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-0046     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 36033..47103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHH29_RS00210 (MHH29_00210) - 36128..36700 (-) 573 WP_017357979.1 signal peptidase I -
  MHH29_RS00215 (MHH29_00215) tapA 36750..37277 (-) 528 WP_056705241.1 amyloid fiber anchoring/assembly protein TapA -
  MHH29_RS00220 (MHH29_00220) - 37530..37856 (+) 327 WP_058336653.1 DUF3889 domain-containing protein -
  MHH29_RS00225 (MHH29_00225) - 37891..38085 (-) 195 WP_024718846.1 YqzE family protein -
  MHH29_RS00230 (MHH29_00230) comGG 38146..38529 (-) 384 WP_342494816.1 competence type IV pilus minor pilin ComGG -
  MHH29_RS00235 (MHH29_00235) comGF 38526..39023 (-) 498 WP_216894677.1 competence type IV pilus minor pilin ComGF -
  MHH29_RS00240 (MHH29_00240) comGE 38965..39279 (-) 315 WP_039166034.1 competence type IV pilus minor pilin ComGE -
  MHH29_RS00245 (MHH29_00245) comGD 39263..39709 (-) 447 WP_096881621.1 competence type IV pilus minor pilin ComGD -
  MHH29_RS00250 (MHH29_00250) comGC 39702..39995 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  MHH29_RS00255 (MHH29_00255) comGB 40012..41052 (-) 1041 WP_024718840.1 competence type IV pilus assembly protein ComGB -
  MHH29_RS00260 (MHH29_00260) comGA 41033..42103 (-) 1071 WP_024718839.1 competence type IV pilus ATPase ComGA Machinery gene
  MHH29_RS00265 (MHH29_00265) - 42561..43433 (-) 873 WP_035390819.1 STAS domain-containing protein -
  MHH29_RS00275 (MHH29_00275) - 43659..44039 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHH29_RS00280 (MHH29_00280) - 44252..44494 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  MHH29_RS00285 (MHH29_00285) - 44543..45181 (-) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  MHH29_RS00290 (MHH29_00290) - 45342..45515 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  MHH29_RS00295 (MHH29_00295) - 45540..45854 (-) 315 WP_056705254.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40366.72 Da        Isoelectric Point: 9.0449

>NTDB_id=986768 MHH29_RS00260 WP_024718839.1 41033..42103(-) (comGA) [Bacillus sp. FSL K6-0046]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEANL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=986768 MHH29_RS00260 WP_024718839.1 41033..42103(-) (comGA) [Bacillus sp. FSL K6-0046]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTGTCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGATCATTGGCT
GTGATGTTAAGACGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCAACTGTAAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACAGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGGAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCTGGCATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCCAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACATTGCAC
GCAAAGAATGCAAAAGGAGCTCTTTATCGAATGCTCGAATTTGGTGTGACGATGAATGAACTCGAACAAACAATGGTTGC
CATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGGTTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAACCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.888

100

0.649

Multiple sequence alignment