Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   R8623_RS09755 Genome accession   NZ_AP026928
Coordinates   1879778..1880719 (-) Length   313 a.a.
NCBI ID   WP_000249555.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PZ900700406     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1874778..1885719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8623_RS09715 (PC0401_18740) comYH 1875272..1876225 (-) 954 WP_000345122.1 class I SAM-dependent methyltransferase Machinery gene
  R8623_RS09720 (PC0401_18750) - 1876286..1876873 (-) 588 WP_000679779.1 class I SAM-dependent methyltransferase -
  R8623_RS09725 (PC0401_18760) comGG/cglG 1876976..1877422 (-) 447 WP_001811371.1 competence type IV pilus minor pilin ComGG Machinery gene
  R8623_RS09730 comGF/cglF 1877400..1877861 (-) 462 WP_000250539.1 competence type IV pilus minor pilin ComGF Machinery gene
  R8623_RS09735 (PC0401_18780) comGE/cglE 1877824..1878126 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R8623_RS09740 (PC0401_18790) comGD/cglD 1878089..1878493 (-) 405 WP_000588030.1 competence type IV pilus minor pilin ComGD Machinery gene
  R8623_RS09745 (PC0401_18800) comGC/cglC 1878486..1878812 (-) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  R8623_RS09750 (PC0401_18810) comGB/cglB 1878814..1879830 (-) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  R8623_RS09755 (PC0401_18820) comGA/cglA/cilD 1879778..1880719 (-) 942 WP_000249555.1 competence type IV pilus ATPase ComGA Machinery gene
  R8623_RS09760 (PC0401_18830) - 1880753..1881157 (-) 405 WP_317649603.1 DUF1033 family protein -
  R8623_RS09765 (PC0401_18840) - 1881308..1882366 (-) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -
  R8623_RS09770 (PC0401_18850) nagA 1882529..1883680 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  R8623_RS09775 (PC0401_18860) - 1883833..1885650 (-) 1818 WP_001220846.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35561.49 Da        Isoelectric Point: 6.0083

>NTDB_id=98623 R8623_RS09755 WP_000249555.1 1879778..1880719(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain PZ900700406]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=98623 R8623_RS09755 WP_000249555.1 1879778..1880719(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain PZ900700406]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCCAAGTT
AGATGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae D39

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae R6

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

98.722

100

0.987
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

97.125

100

0.971
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364

Multiple sequence alignment