Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   MHI58_RS12070 Genome accession   NZ_CP152035
Coordinates   2338766..2339059 (-) Length   97 a.a.
NCBI ID   WP_008342186.1    Uniprot ID   A0A5K1NAM4
Organism   Bacillus sp. FSL K6-2869     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2333766..2344059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI58_RS12020 (MHI58_12020) sinR 2333930..2334265 (+) 336 WP_017357978.1 transcriptional regulator SinR Regulator
  MHI58_RS12025 (MHI58_12025) tasA 2334333..2335133 (-) 801 WP_008342195.1 biofilm matrix protein TasA -
  MHI58_RS12030 (MHI58_12030) sipW 2335192..2335764 (-) 573 WP_017357979.1 signal peptidase I SipW -
  MHI58_RS12035 (MHI58_12035) tapA 2335814..2336341 (-) 528 WP_045034369.1 amyloid fiber anchoring/assembly protein TapA -
  MHI58_RS12040 (MHI58_12040) - 2336594..2336920 (+) 327 WP_045034370.1 DUF3889 domain-containing protein -
  MHI58_RS12045 (MHI58_12045) - 2336955..2337149 (-) 195 WP_008342191.1 YqzE family protein -
  MHI58_RS12050 (MHI58_12050) comGG 2337210..2337593 (-) 384 WP_045034371.1 competence type IV pilus minor pilin ComGG -
  MHI58_RS12055 (MHI58_12055) comGF 2337590..2338087 (-) 498 WP_045034372.1 competence type IV pilus minor pilin ComGF -
  MHI58_RS12060 (MHI58_12060) comGE 2338029..2338343 (-) 315 WP_041506956.1 competence type IV pilus minor pilin ComGE -
  MHI58_RS12065 (MHI58_12065) comGD 2338327..2338773 (-) 447 WP_045034373.1 competence type IV pilus minor pilin ComGD -
  MHI58_RS12070 (MHI58_12070) comGC 2338766..2339059 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  MHI58_RS12075 (MHI58_12075) comGB 2339076..2340116 (-) 1041 WP_041506954.1 competence type IV pilus assembly protein ComGB -
  MHI58_RS12080 (MHI58_12080) comGA 2340097..2341167 (-) 1071 WP_024718839.1 competence type IV pilus ATPase ComGA Machinery gene
  MHI58_RS12085 (MHI58_12085) - 2341624..2342496 (-) 873 WP_041506953.1 STAS domain-containing protein -
  MHI58_RS12095 (MHI58_12095) - 2342721..2343101 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHI58_RS12100 (MHI58_12100) - 2343314..2343556 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10728.62 Da        Isoelectric Point: 6.7081

>NTDB_id=986071 MHI58_RS12070 WP_008342186.1 2338766..2339059(-) (comGC) [Bacillus sp. FSL K6-2869]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQSIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIFIQNGDVKNE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=986071 MHI58_RS12070 WP_008342186.1 2338766..2339059(-) (comGC) [Bacillus sp. FSL K6-2869]
ATGAATGATAAAGGATTTACACTGTTAGAAATGTTAATTGTCATGCTTGTGATCTCCATTCTTTTATTGATCACCATTCC
AAATATCACTCGGCATCATCAAAGCATTCAAGCAAAGGGCTGTGAAGGGCTGAAATCAATGGTGAGTACACAGGTAATGG
CATATGAATTAGAGCATGACGGCAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGCTATGTCAAAAAAGGATTAACT
TGTCCGAATGGAAAGGCAATTTTCATTCAAAACGGGGATGTGAAAAATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5K1NAM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67
  comGC Staphylococcus aureus MW2

41.304

94.845

0.392
  comGC Staphylococcus aureus N315

41.304

94.845

0.392

Multiple sequence alignment