Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MHI58_RS12080 Genome accession   NZ_CP152035
Coordinates   2340097..2341167 (-) Length   356 a.a.
NCBI ID   WP_024718839.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-2869     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2335097..2346167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI58_RS12030 (MHI58_12030) sipW 2335192..2335764 (-) 573 WP_017357979.1 signal peptidase I SipW -
  MHI58_RS12035 (MHI58_12035) tapA 2335814..2336341 (-) 528 WP_045034369.1 amyloid fiber anchoring/assembly protein TapA -
  MHI58_RS12040 (MHI58_12040) - 2336594..2336920 (+) 327 WP_045034370.1 DUF3889 domain-containing protein -
  MHI58_RS12045 (MHI58_12045) - 2336955..2337149 (-) 195 WP_008342191.1 YqzE family protein -
  MHI58_RS12050 (MHI58_12050) comGG 2337210..2337593 (-) 384 WP_045034371.1 competence type IV pilus minor pilin ComGG -
  MHI58_RS12055 (MHI58_12055) comGF 2337590..2338087 (-) 498 WP_045034372.1 competence type IV pilus minor pilin ComGF -
  MHI58_RS12060 (MHI58_12060) comGE 2338029..2338343 (-) 315 WP_041506956.1 competence type IV pilus minor pilin ComGE -
  MHI58_RS12065 (MHI58_12065) comGD 2338327..2338773 (-) 447 WP_045034373.1 competence type IV pilus minor pilin ComGD -
  MHI58_RS12070 (MHI58_12070) comGC 2338766..2339059 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  MHI58_RS12075 (MHI58_12075) comGB 2339076..2340116 (-) 1041 WP_041506954.1 competence type IV pilus assembly protein ComGB -
  MHI58_RS12080 (MHI58_12080) comGA 2340097..2341167 (-) 1071 WP_024718839.1 competence type IV pilus ATPase ComGA Machinery gene
  MHI58_RS12085 (MHI58_12085) - 2341624..2342496 (-) 873 WP_041506953.1 STAS domain-containing protein -
  MHI58_RS12095 (MHI58_12095) - 2342721..2343101 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHI58_RS12100 (MHI58_12100) - 2343314..2343556 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  MHI58_RS12105 (MHI58_12105) - 2343605..2344243 (-) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  MHI58_RS12110 (MHI58_12110) - 2344404..2344577 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  MHI58_RS12115 (MHI58_12115) - 2344602..2344916 (-) 315 WP_017367597.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40366.72 Da        Isoelectric Point: 9.0449

>NTDB_id=986072 MHI58_RS12080 WP_024718839.1 2340097..2341167(-) (comGA) [Bacillus sp. FSL K6-2869]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEANL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=986072 MHI58_RS12080 WP_024718839.1 2340097..2341167(-) (comGA) [Bacillus sp. FSL K6-2869]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGATCATTGGCT
GTGATGCTAAGACGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCAACTGTAAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACAGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGGAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGTTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCAGAAACAGCGAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACACTGCAC
GCAAAGAATGCAAAAGGAGCTCTTTATCGAATGCTCGAATTTGGTGTGACGATGAATGAACTCGAACAAACAATGGTTGC
CATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGGTTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAACCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.888

100

0.649

Multiple sequence alignment