Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MHI41_RS16475 Genome accession   NZ_CP152023
Coordinates   3205105..3206232 (-) Length   375 a.a.
NCBI ID   WP_342494316.1    Uniprot ID   -
Organism   Bacillus sp. FSL M7-0307     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3200105..3211232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI41_RS16440 (MHI41_16440) flgL 3200288..3201199 (-) 912 WP_017368141.1 flagellar hook-associated protein FlgL -
  MHI41_RS16445 (MHI41_16445) flgK 3201208..3202728 (-) 1521 WP_017368142.1 flagellar hook-associated protein FlgK -
  MHI41_RS16450 (MHI41_16450) - 3202744..3203226 (-) 483 WP_007499977.1 flagellar protein FlgN -
  MHI41_RS16455 (MHI41_16455) flgM 3203245..3203508 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  MHI41_RS16460 (MHI41_16460) - 3203588..3204007 (-) 420 WP_017357864.1 TIGR03826 family flagellar region protein -
  MHI41_RS16465 (MHI41_16465) - 3204064..3204786 (-) 723 WP_342494315.1 ComF family protein -
  MHI41_RS16470 (MHI41_16470) - 3204747..3205040 (-) 294 WP_035701582.1 competence protein ComFB -
  MHI41_RS16475 (MHI41_16475) comFA 3205105..3206232 (-) 1128 WP_342494316.1 DEAD/DEAH box helicase Machinery gene
  MHI41_RS16480 (MHI41_16480) - 3206604..3207446 (-) 843 WP_060832692.1 DegV family protein -
  MHI41_RS16485 (MHI41_16485) degU 3207658..3208347 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  MHI41_RS16490 (MHI41_16490) degS 3208412..3209587 (-) 1176 WP_035389673.1 sensor histidine kinase Regulator
  MHI41_RS16495 (MHI41_16495) - 3209809..3210477 (+) 669 WP_017357860.1 YigZ family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 43004.05 Da        Isoelectric Point: 10.4547

>NTDB_id=985411 MHI41_RS16475 WP_342494316.1 3205105..3206232(-) (comFA) [Bacillus sp. FSL M7-0307]
MLGKVSECGFLYEWTGPQKKETRQVELTWQGELSKGQKRASDRLVEAIQNKFDLLVWAVCGAGKTEVLFQGIKYALNNGM
SVCIATPRTDVVLELEPRMRKAFQGLNIAALYGGSSQRFQMAPLMIATTHQLMRYKNAFDVLIVDEVDAFPYSMDERLQF
AVLKSMKKTGVRIYLSATPSKKMQKDVARRQLEAIKIPLRFHQKPLPVPTFQWVGDWKKRLKRNRLPASTMNWLHEHITK
KKRVLLFVPSISTMKKVTHILQVQDVNVQGVSADDQERKQKVQHFRDHRYDVLVTTTILERGVTIQDVQVGVLGAESPIF
TESALVQISGRAGRHPDFFYGDVYFFHFGLTRSMKQAKKHIVKMNNTAEKEFSEK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=985411 MHI41_RS16475 WP_342494316.1 3205105..3206232(-) (comFA) [Bacillus sp. FSL M7-0307]
ATGCTGGGAAAAGTAAGCGAATGTGGCTTTCTCTACGAATGGACGGGTCCACAAAAGAAAGAAACAAGACAAGTTGAATT
AACATGGCAGGGAGAGCTTTCTAAAGGGCAAAAGAGAGCGTCCGATAGGCTAGTAGAAGCAATCCAAAACAAATTTGATC
TGCTCGTTTGGGCTGTTTGTGGTGCGGGGAAAACGGAGGTGCTTTTTCAGGGAATAAAATATGCGCTAAATAACGGAATG
AGTGTTTGTATTGCGACGCCTAGAACCGATGTTGTACTTGAGCTTGAACCTCGAATGAGAAAGGCATTTCAAGGACTGAA
TATAGCTGCACTTTATGGCGGGAGTTCTCAAAGATTTCAAATGGCACCGCTTATGATCGCTACAACCCATCAGCTAATGA
GATATAAAAACGCCTTTGATGTCCTCATTGTAGACGAAGTCGATGCTTTTCCTTATTCAATGGATGAACGGCTTCAGTTT
GCTGTTTTAAAGTCAATGAAAAAAACAGGAGTTAGAATTTACTTAAGTGCCACACCATCTAAAAAAATGCAAAAGGATGT
TGCTCGTAGACAATTAGAAGCGATCAAAATTCCTCTGCGCTTCCACCAAAAACCGTTGCCTGTCCCAACATTTCAATGGG
TTGGAGATTGGAAAAAGAGATTAAAAAGAAATCGGCTTCCAGCATCTACAATGAACTGGCTGCATGAACATATCACAAAG
AAAAAAAGAGTGTTACTCTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACTCATATTCTTCAAGTTCAAGATGTAAA
TGTACAGGGTGTTTCTGCGGATGATCAAGAAAGGAAGCAAAAAGTCCAGCATTTCAGAGATCATAGGTACGATGTATTGG
TTACAACAACTATTCTAGAAAGAGGCGTTACCATTCAAGATGTTCAAGTGGGCGTATTAGGTGCGGAATCTCCTATTTTT
ACAGAAAGTGCACTTGTCCAAATTTCTGGTAGAGCAGGTAGACATCCAGATTTTTTTTACGGAGACGTGTACTTTTTTCA
TTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAACATATCGTAAAAATGAATAATACAGCTGAAAAAGAGTTTTCTG
AAAAATAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(236-335)

Type III restriction enzyme, res subunit (ResIII)

(32-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

55.676

98.667

0.549
  comFA/cflA Streptococcus pneumoniae Rx1

41.525

94.4

0.392
  comFA/cflA Streptococcus pneumoniae D39

41.525

94.4

0.392
  comFA/cflA Streptococcus pneumoniae R6

41.525

94.4

0.392
  comFA/cflA Streptococcus pneumoniae TIGR4

41.243

94.4

0.389
  comFA/cflA Streptococcus mitis NCTC 12261

40.96

94.4

0.387
  comFA Latilactobacillus sakei subsp. sakei 23K

38.965

97.867

0.381
  comFA/cflA Streptococcus mitis SK321

39.831

94.4

0.376
  comFA Lactococcus lactis subsp. cremoris KW2

37.705

97.6

0.368

Multiple sequence alignment