Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   MHI41_RS11405 Genome accession   NZ_CP152023
Coordinates   2232891..2233184 (-) Length   97 a.a.
NCBI ID   WP_008342186.1    Uniprot ID   A0A5K1NAM4
Organism   Bacillus sp. FSL M7-0307     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2227891..2238184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI41_RS11355 (MHI41_11355) sinR 2228050..2228385 (+) 336 WP_017357978.1 helix-turn-helix domain-containing protein Regulator
  MHI41_RS11360 (MHI41_11360) - 2228453..2229253 (-) 801 WP_035390805.1 TasA family protein -
  MHI41_RS11365 (MHI41_11365) - 2229312..2229884 (-) 573 WP_041091749.1 signal peptidase I -
  MHI41_RS11370 (MHI41_11370) tapA 2229934..2230461 (-) 528 WP_046527018.1 amyloid fiber anchoring/assembly protein TapA -
  MHI41_RS11375 (MHI41_11375) - 2230714..2231040 (+) 327 WP_017367590.1 DUF3889 domain-containing protein -
  MHI41_RS11380 (MHI41_11380) - 2231074..2231274 (-) 201 WP_046343970.1 YqzE family protein -
  MHI41_RS11385 (MHI41_11385) comGG 2231335..2231718 (-) 384 WP_272511702.1 competence type IV pilus minor pilin ComGG -
  MHI41_RS11390 (MHI41_11390) comGF 2231715..2232212 (-) 498 WP_065460694.1 competence type IV pilus minor pilin ComGF -
  MHI41_RS11395 (MHI41_11395) comGE 2232154..2232468 (-) 315 WP_065460696.1 competence type IV pilus minor pilin ComGE -
  MHI41_RS11400 (MHI41_11400) comGD 2232452..2232898 (-) 447 WP_073413947.1 competence type IV pilus minor pilin ComGD -
  MHI41_RS11405 (MHI41_11405) comGC 2232891..2233184 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  MHI41_RS11410 (MHI41_11410) comGB 2233201..2234241 (-) 1041 WP_282833861.1 competence type IV pilus assembly protein ComGB -
  MHI41_RS11415 (MHI41_11415) comGA 2234222..2235292 (-) 1071 WP_282833860.1 competence type IV pilus ATPase ComGA Machinery gene
  MHI41_RS11420 (MHI41_11420) - 2235757..2236629 (-) 873 WP_035390819.1 STAS domain-containing protein -
  MHI41_RS11430 (MHI41_11430) - 2236855..2237235 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHI41_RS11435 (MHI41_11435) - 2237448..2237690 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10728.62 Da        Isoelectric Point: 6.7081

>NTDB_id=985394 MHI41_RS11405 WP_008342186.1 2232891..2233184(-) (comGC) [Bacillus sp. FSL M7-0307]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQSIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIFIQNGDVKNE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=985394 MHI41_RS11405 WP_008342186.1 2232891..2233184(-) (comGC) [Bacillus sp. FSL M7-0307]
ATGAATGATAAAGGATTTACACTGTTAGAAATGTTAATTGTCATGCTTGTGATCTCCATTCTTTTATTGATCACCATTCC
AAATATCACTCGGCATCATCAAAGCATTCAAGCAAAGGGCTGTGAAGGGCTGAAATCAATGGTGAGTACACAGGTAATGG
CATATGAATTAGAGCATGATGGCAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGCTATGTCAAAAAGGGATTAACT
TGTCCGAATGGAAAGGCAATTTTCATTCAAAATGGGGATGTGAAAAATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5K1NAM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67
  comGC Staphylococcus aureus MW2

41.304

94.845

0.392
  comGC Staphylococcus aureus N315

41.304

94.845

0.392

Multiple sequence alignment