Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGC   Type   Machinery gene
Locus tag   MHI08_RS11325 Genome accession   NZ_CP152022
Coordinates   2213591..2213884 (-) Length   97 a.a.
NCBI ID   WP_024422977.1    Uniprot ID   A0A0M2EIN6
Organism   Bacillus sp. FSL M7-0791     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2208591..2218884
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI08_RS11275 (MHI08_11275) sinR 2208758..2209093 (+) 336 WP_024422969.1 transcriptional regulator SinR Regulator
  MHI08_RS11280 (MHI08_11280) - 2209161..2209961 (-) 801 WP_098677431.1 TasA family protein -
  MHI08_RS11285 (MHI08_11285) - 2210016..2210588 (-) 573 WP_024422971.1 signal peptidase I -
  MHI08_RS11290 (MHI08_11290) tapA 2210641..2211168 (-) 528 WP_234107214.1 amyloid fiber anchoring/assembly protein TapA -
  MHI08_RS11295 (MHI08_11295) - 2211468..2211746 (+) 279 WP_268415647.1 DUF3889 domain-containing protein -
  MHI08_RS11300 (MHI08_11300) - 2211781..2211975 (-) 195 WP_056767455.1 YqzE family protein -
  MHI08_RS11305 (MHI08_11305) comGG 2212035..2212418 (-) 384 WP_103131951.1 competence type IV pilus minor pilin ComGG -
  MHI08_RS11310 (MHI08_11310) comGF 2212415..2212942 (-) 528 WP_311164128.1 competence type IV pilus minor pilin ComGF -
  MHI08_RS11315 (MHI08_11315) comGE 2212854..2213168 (-) 315 WP_087978326.1 competence type IV pilus minor pilin ComGE -
  MHI08_RS11320 (MHI08_11320) comGD 2213152..2213598 (-) 447 WP_342499627.1 competence type IV pilus minor pilin ComGD -
  MHI08_RS11325 (MHI08_11325) comGC 2213591..2213884 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  MHI08_RS11330 (MHI08_11330) comGB 2213901..2214941 (-) 1041 WP_024422978.1 competence type IV pilus assembly protein ComGB -
  MHI08_RS11335 (MHI08_11335) comGA 2214922..2215992 (-) 1071 WP_041117979.1 competence type IV pilus ATPase ComGA Machinery gene
  MHI08_RS11340 (MHI08_11340) - 2216170..2217045 (-) 876 WP_024422979.1 STAS domain-containing protein -
  MHI08_RS11350 (MHI08_11350) - 2217269..2217649 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHI08_RS11355 (MHI08_11355) - 2217862..2218104 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  MHI08_RS11360 (MHI08_11360) - 2218152..2218790 (-) 639 WP_056767335.1 MBL fold metallo-hydrolase -

Sequence


Protein


Download         Length: 97 a.a.        Molecular weight: 10686.62 Da        Isoelectric Point: 7.3218

>NTDB_id=985333 MHI08_RS11325 WP_024422977.1 2213591..2213884(-) (comGC) [Bacillus sp. FSL M7-0791]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQAIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIVIQNGNVKHE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=985333 MHI08_RS11325 WP_024422977.1 2213591..2213884(-) (comGC) [Bacillus sp. FSL M7-0791]
ATGAATGATAAAGGATTTACCCTGTTAGAAATGTTAATAGTCATGCTCGTAATATCCATTCTTTTACTCATTACCATACC
GAATATCACACGTCATCACCAAGCTATTCAGGCAAAGGGCTGTGAAGGGCTTAAATCAATGGTCAGCACACAAGTGATGG
CATATGAATTGGAGCATGATGGGAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGTTATGTCAAAAAGGGGTTAACT
TGTCCTAATGGAAAAGCGATTGTCATTCAAAACGGCAACGTGAAGCATGAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0M2EIN6

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67

  comGC Staphylococcus aureus MW2

42.222

92.784

0.392

  comGC Staphylococcus aureus N315

42.222

92.784

0.392


Multiple sequence alignment