Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MHI08_RS11335 Genome accession   NZ_CP152022
Coordinates   2214922..2215992 (-) Length   356 a.a.
NCBI ID   WP_041117979.1    Uniprot ID   -
Organism   Bacillus sp. FSL M7-0791     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2209922..2220992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI08_RS11285 (MHI08_11285) - 2210016..2210588 (-) 573 WP_024422971.1 signal peptidase I -
  MHI08_RS11290 (MHI08_11290) tapA 2210641..2211168 (-) 528 WP_234107214.1 amyloid fiber anchoring/assembly protein TapA -
  MHI08_RS11295 (MHI08_11295) - 2211468..2211746 (+) 279 WP_268415647.1 DUF3889 domain-containing protein -
  MHI08_RS11300 (MHI08_11300) - 2211781..2211975 (-) 195 WP_056767455.1 YqzE family protein -
  MHI08_RS11305 (MHI08_11305) comGG 2212035..2212418 (-) 384 WP_103131951.1 competence type IV pilus minor pilin ComGG -
  MHI08_RS11310 (MHI08_11310) comGF 2212415..2212942 (-) 528 WP_311164128.1 competence type IV pilus minor pilin ComGF -
  MHI08_RS11315 (MHI08_11315) comGE 2212854..2213168 (-) 315 WP_087978326.1 competence type IV pilus minor pilin ComGE -
  MHI08_RS11320 (MHI08_11320) comGD 2213152..2213598 (-) 447 WP_342499627.1 competence type IV pilus minor pilin ComGD -
  MHI08_RS11325 (MHI08_11325) comGC 2213591..2213884 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  MHI08_RS11330 (MHI08_11330) comGB 2213901..2214941 (-) 1041 WP_024422978.1 competence type IV pilus assembly protein ComGB -
  MHI08_RS11335 (MHI08_11335) comGA 2214922..2215992 (-) 1071 WP_041117979.1 competence type IV pilus ATPase ComGA Machinery gene
  MHI08_RS11340 (MHI08_11340) - 2216170..2217045 (-) 876 WP_024422979.1 STAS domain-containing protein -
  MHI08_RS11350 (MHI08_11350) - 2217269..2217649 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHI08_RS11355 (MHI08_11355) - 2217862..2218104 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  MHI08_RS11360 (MHI08_11360) - 2218152..2218790 (-) 639 WP_056767335.1 MBL fold metallo-hydrolase -
  MHI08_RS11365 (MHI08_11365) - 2218948..2219121 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  MHI08_RS11370 (MHI08_11370) - 2219147..2219461 (-) 315 WP_024427984.1 MTH1187 family thiamine-binding protein -
  MHI08_RS11375 (MHI08_11375) - 2219538..2220791 (-) 1254 WP_056767338.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40398.79 Da        Isoelectric Point: 9.4061

>NTDB_id=985334 MHI08_RS11335 WP_041117979.1 2214922..2215992(-) (comGA) [Bacillus sp. FSL M7-0791]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRNGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDVIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGERCQLYCKLNRPIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=985334 MHI08_RS11335 WP_041117979.1 2214922..2215992(-) (comGA) [Bacillus sp. FSL M7-0791]
TTGTATGGGATTGAATATTTAAGACAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCATTTCGTGTTGATTCAGATTTAATCCAGCAGCGAACGATTGATAAAAAAAGCGGAG
AGCGGCTTATTGCACATTTTAAGTTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGGCCGCAAAATGGCTCACTAGCT
GTGATGCTAAGAAACGGTCAAGTGTTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGTTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATTAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGATTATTATCCTTTTTAA
ACCACTCGCATGGACTCATTTTATTTACTGGGCCAACCAATTCGGGGAAAACAACAACCCTCTATTCACTGATCCAGTTT
GCAAAAAAGAATTTCAACCGAAATATTATTACACTTGAAGATCCTGTCGAAACAAGGAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCCGGCATCACATATGCCGCAGGATTACGCGCTATTTTAAGACATGATCCAGATGTGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCCAGAACGGCGATTAGAGCAGCGCTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTTGAGCAAACGATGGTTGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAAGATGTCAGCTTTACTGTAAATTAAATCGAC
CGATCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTCGGCTATTTATCAAAAAACACTTACCA
TCGCTGGGTTTATGAAGAAGCGAGTCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.689

99.438

0.643

Multiple sequence alignment