Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MKY11_RS17035 Genome accession   NZ_CP152000
Coordinates   3264831..3265958 (-) Length   375 a.a.
NCBI ID   WP_274660532.1    Uniprot ID   -
Organism   Bacillus sp. FSL W7-1085     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3259831..3270958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY11_RS16995 (MKY11_16995) flgL 3260014..3260925 (-) 912 WP_008348257.1 flagellar hook-associated protein FlgL -
  MKY11_RS17000 (MKY11_17000) flgK 3260934..3262454 (-) 1521 WP_058336981.1 flagellar hook-associated protein FlgK -
  MKY11_RS17005 (MKY11_17005) - 3262470..3262952 (-) 483 WP_007499977.1 flagellar protein FlgN -
  MKY11_RS17010 (MKY11_17010) flgM 3262971..3263234 (-) 264 WP_274660533.1 flagellar biosynthesis anti-sigma factor FlgM -
  MKY11_RS17015 (MKY11_17015) - 3263314..3263733 (-) 420 WP_017357864.1 TIGR03826 family flagellar region protein -
  MKY11_RS17020 (MKY11_17020) comFC 3263790..3264257 (-) 468 WP_034647592.1 ComF family protein Machinery gene
  MKY11_RS17025 (MKY11_17025) - 3264309..3264512 (-) 204 Protein_3298 hypothetical protein -
  MKY11_RS17030 (MKY11_17030) - 3264473..3264766 (-) 294 WP_008348267.1 competence protein ComFB -
  MKY11_RS17035 (MKY11_17035) comFA 3264831..3265958 (-) 1128 WP_274660532.1 DEAD/DEAH box helicase Machinery gene
  MKY11_RS17040 (MKY11_17040) - 3266330..3267172 (-) 843 WP_024719242.1 DegV family protein -
  MKY11_RS17045 (MKY11_17045) degU 3267384..3268073 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  MKY11_RS17050 (MKY11_17050) degS 3268138..3269313 (-) 1176 WP_024719243.1 sensor histidine kinase Regulator
  MKY11_RS17055 (MKY11_17055) - 3269535..3270203 (+) 669 WP_017357860.1 YigZ family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42924.97 Da        Isoelectric Point: 10.3931

>NTDB_id=984316 MKY11_RS17035 WP_274660532.1 3264831..3265958(-) (comFA) [Bacillus sp. FSL W7-1085]
MLGKVSECGFLYEWTGPQKKETRQIELTWQGELSKGQKRASDRLVEAIQNKFDLLVWAVCGAGKTEVLFHGIKYALNNGM
SVCIATPRTDVVLELEPRMRKAFQGLNIAALYGGSSQRFQMAPLMIATTHQLMRYKNAFDVLIVDEVDAFPYSMDERLQF
AVLKSMKKTGVRIYLSATPSKKMQKDVARRQLEAIKIPLRFHQKPLPVPTFQWVGDWKKKLKRNRLPASTMNWLHEHITK
KKRVLLFVPSISTMKKVTHILQVQDVNVQCVSADDQGRKQKVQHFRDHRYDVLVTTTILERGVTIQDVQVGVLGAESPIF
TESALVQISGRAGRHPDFFNGDVYFFHFGLTRSMKQAKKHIVKMNNTAEEEFSEK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=984316 MKY11_RS17035 WP_274660532.1 3264831..3265958(-) (comFA) [Bacillus sp. FSL W7-1085]
ATGCTGGGAAAAGTAAGCGAATGTGGCTTTCTCTACGAATGGACGGGTCCACAAAAGAAAGAAACAAGACAAATTGAATT
AACATGGCAGGGAGAGCTTTCTAAAGGGCAAAAGAGAGCGTCCGATAGGCTAGTAGAAGCAATTCAAAACAAATTTGATC
TGCTCGTTTGGGCTGTTTGTGGTGCGGGGAAAACGGAGGTGCTTTTTCATGGTATAAAATATGCGCTAAATAACGGAATG
AGTGTTTGTATTGCGACGCCTAGAACCGATGTTGTACTTGAGCTCGAACCTCGAATGAGAAAGGCATTTCAAGGACTGAA
TATAGCTGCACTTTATGGCGGGAGTTCTCAAAGATTTCAAATGGCACCGCTTATGATCGCTACAACCCATCAGCTAATGA
GATATAAAAACGCCTTTGATGTCCTCATTGTAGACGAAGTCGATGCATTTCCTTATTCAATGGATGAACGGCTTCAGTTT
GCTGTTTTAAAATCAATGAAAAAAACAGGAGTTAGAATTTACTTAAGTGCCACACCATCTAAAAAAATGCAAAAGGATGT
TGCTCGTAGACAATTAGAAGCGATCAAAATTCCTCTGCGCTTCCACCAAAAACCGTTGCCTGTCCCAACTTTTCAATGGG
TTGGAGATTGGAAAAAGAAATTAAAAAGAAATCGGCTTCCAGCATCTACAATGAACTGGCTGCATGAACATATCACAAAG
AAAAAAAGAGTGTTACTCTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACTCATATTCTTCAAGTTCAAGATGTAAA
TGTACAGTGTGTTTCTGCGGATGATCAAGGAAGGAAACAAAAAGTCCAGCATTTCAGAGATCATAGATACGATGTATTGG
TTACAACAACTATTCTAGAAAGAGGCGTTACTATTCAAGATGTTCAAGTGGGCGTATTAGGTGCGGAATCTCCTATTTTT
ACAGAAAGTGCACTTGTCCAAATTTCTGGTAGAGCAGGGAGACATCCAGATTTTTTTAACGGAGACGTGTACTTTTTTCA
TTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAACATATCGTAAAAATGAATAATACAGCTGAAGAAGAGTTTTCTG
AAAAATAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(32-180)

Helicase conserved C-terminal domain (Helicase_C)

(236-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

56.25

98.133

0.552
  comFA/cflA Streptococcus pneumoniae Rx1

41.243

94.4

0.389
  comFA/cflA Streptococcus pneumoniae D39

41.243

94.4

0.389
  comFA/cflA Streptococcus pneumoniae R6

41.243

94.4

0.389
  comFA/cflA Streptococcus pneumoniae TIGR4

40.96

94.4

0.387
  comFA/cflA Streptococcus mitis NCTC 12261

40.678

94.4

0.384
  comFA Latilactobacillus sakei subsp. sakei 23K

38.587

98.133

0.379
  comFA/cflA Streptococcus mitis SK321

39.548

94.4

0.373
  comFA Lactococcus lactis subsp. cremoris KW2

37.705

97.6

0.368

Multiple sequence alignment