Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   NST73_RS11675 Genome accession   NZ_CP151989
Coordinates   2271211..2271504 (-) Length   97 a.a.
NCBI ID   WP_008342186.1    Uniprot ID   A0A5K1NAM4
Organism   Bacillus sp. FSL W7-1034     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2266211..2276504
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST73_RS11625 (NST73_11625) sinR 2266375..2266710 (+) 336 WP_017357978.1 helix-turn-helix domain-containing protein Regulator
  NST73_RS11630 (NST73_11630) - 2266778..2267578 (-) 801 WP_008342195.1 TasA family protein -
  NST73_RS11635 (NST73_11635) - 2267637..2268209 (-) 573 WP_017357979.1 signal peptidase I -
  NST73_RS11640 (NST73_11640) tapA 2268259..2268786 (-) 528 WP_342499103.1 amyloid fiber anchoring/assembly protein TapA -
  NST73_RS11645 (NST73_11645) - 2269039..2269365 (+) 327 WP_039166025.1 DUF3889 domain-containing protein -
  NST73_RS11650 (NST73_11650) - 2269400..2269594 (-) 195 WP_008342191.1 YqzE family protein -
  NST73_RS11655 (NST73_11655) comGG 2269655..2270038 (-) 384 WP_342499104.1 competence type IV pilus minor pilin ComGG -
  NST73_RS11660 (NST73_11660) comGF 2270035..2270532 (-) 498 WP_034647459.1 competence type IV pilus minor pilin ComGF -
  NST73_RS11665 (NST73_11665) comGE 2270474..2270788 (-) 315 WP_039166034.1 competence type IV pilus minor pilin ComGE -
  NST73_RS11670 (NST73_11670) comGD 2270772..2271218 (-) 447 WP_096881621.1 competence type IV pilus minor pilin ComGD -
  NST73_RS11675 (NST73_11675) comGC 2271211..2271504 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  NST73_RS11680 (NST73_11680) comGB 2271521..2272561 (-) 1041 WP_041506954.1 competence type IV pilus assembly protein ComGB -
  NST73_RS11685 (NST73_11685) comGA 2272542..2273612 (-) 1071 WP_035390817.1 competence type IV pilus ATPase ComGA Machinery gene
  NST73_RS11690 (NST73_11690) - 2274071..2274943 (-) 873 WP_024718838.1 STAS domain-containing protein -
  NST73_RS11700 (NST73_11700) - 2275169..2275549 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NST73_RS11705 (NST73_11705) - 2275762..2276004 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10728.62 Da        Isoelectric Point: 6.7081

>NTDB_id=983744 NST73_RS11675 WP_008342186.1 2271211..2271504(-) (comGC) [Bacillus sp. FSL W7-1034]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQSIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIFIQNGDVKNE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=983744 NST73_RS11675 WP_008342186.1 2271211..2271504(-) (comGC) [Bacillus sp. FSL W7-1034]
ATGAATGATAAAGGATTTACACTGTTAGAAATGTTAATTGTCATGCTTGTGATCTCCATTCTTTTATTGATCACCATTCC
AAATATCACTCGGCATCATCAAAGCATTCAAGCAAAGGGCTGTGAAGGGCTGAAATCAATGGTGAGTACACAGGTAATGG
CATATGAATTAGAGCATGACGGCAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGCTATGTCAAAAAAGGATTAACT
TGTCCAAATGGAAAGGCAATTTTCATTCAAAACGGGGATGTGAAAAATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5K1NAM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67
  comGC Staphylococcus aureus MW2

41.304

94.845

0.392
  comGC Staphylococcus aureus N315

41.304

94.845

0.392

Multiple sequence alignment