Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NST73_RS11685 Genome accession   NZ_CP151989
Coordinates   2272542..2273612 (-) Length   356 a.a.
NCBI ID   WP_035390817.1    Uniprot ID   -
Organism   Bacillus sp. FSL W7-1034     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2267542..2278612
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST73_RS11635 (NST73_11635) - 2267637..2268209 (-) 573 WP_017357979.1 signal peptidase I -
  NST73_RS11640 (NST73_11640) tapA 2268259..2268786 (-) 528 WP_342499103.1 amyloid fiber anchoring/assembly protein TapA -
  NST73_RS11645 (NST73_11645) - 2269039..2269365 (+) 327 WP_039166025.1 DUF3889 domain-containing protein -
  NST73_RS11650 (NST73_11650) - 2269400..2269594 (-) 195 WP_008342191.1 YqzE family protein -
  NST73_RS11655 (NST73_11655) comGG 2269655..2270038 (-) 384 WP_342499104.1 competence type IV pilus minor pilin ComGG -
  NST73_RS11660 (NST73_11660) comGF 2270035..2270532 (-) 498 WP_034647459.1 competence type IV pilus minor pilin ComGF -
  NST73_RS11665 (NST73_11665) comGE 2270474..2270788 (-) 315 WP_039166034.1 competence type IV pilus minor pilin ComGE -
  NST73_RS11670 (NST73_11670) comGD 2270772..2271218 (-) 447 WP_096881621.1 competence type IV pilus minor pilin ComGD -
  NST73_RS11675 (NST73_11675) comGC 2271211..2271504 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  NST73_RS11680 (NST73_11680) comGB 2271521..2272561 (-) 1041 WP_041506954.1 competence type IV pilus assembly protein ComGB -
  NST73_RS11685 (NST73_11685) comGA 2272542..2273612 (-) 1071 WP_035390817.1 competence type IV pilus ATPase ComGA Machinery gene
  NST73_RS11690 (NST73_11690) - 2274071..2274943 (-) 873 WP_024718838.1 STAS domain-containing protein -
  NST73_RS11700 (NST73_11700) - 2275169..2275549 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NST73_RS11705 (NST73_11705) - 2275762..2276004 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  NST73_RS11710 (NST73_11710) - 2276053..2276691 (-) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  NST73_RS11715 (NST73_11715) - 2276852..2277025 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  NST73_RS11720 (NST73_11720) - 2277050..2277364 (-) 315 WP_017367597.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40396.75 Da        Isoelectric Point: 9.0449

>NTDB_id=983745 NST73_RS11685 WP_035390817.1 2272542..2273612(-) (comGA) [Bacillus sp. FSL W7-1034]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASTRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEANL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=983745 NST73_RS11685 WP_035390817.1 2272542..2273612(-) (comGA) [Bacillus sp. FSL W7-1034]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGATCATTGGCT
GTGATGTTAAGACGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCAACTGTAAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAACGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACAGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGGAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCCAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACATTGCAC
GCAAAGAATGCAAAAGGAGCTCTTTATCGAATGCTCGAATTTGGTGTGACGATGAATGAACTCGAACAAACAATGGTTGC
TATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGGTTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAACCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.607

100

0.646

Multiple sequence alignment