Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   NST09_RS16520 Genome accession   NZ_CP151988
Coordinates   3186535..3187662 (-) Length   375 a.a.
NCBI ID   WP_234898350.1    Uniprot ID   -
Organism   Bacillus sp. FSL W7-1346     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3181535..3192662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST09_RS16485 (NST09_16485) flgL 3181718..3182629 (-) 912 WP_008348257.1 flagellar hook-associated protein FlgL -
  NST09_RS16490 (NST09_16490) flgK 3182638..3184158 (-) 1521 WP_216942853.1 flagellar hook-associated protein FlgK -
  NST09_RS16495 (NST09_16495) - 3184174..3184656 (-) 483 WP_007499977.1 flagellar protein FlgN -
  NST09_RS16500 (NST09_16500) flgM 3184675..3184938 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  NST09_RS16505 (NST09_16505) - 3185018..3185437 (-) 420 WP_017357864.1 TIGR03826 family flagellar region protein -
  NST09_RS16510 (NST09_16510) comFC 3185494..3186180 (-) 687 WP_216942854.1 ComF family protein Machinery gene
  NST09_RS16515 (NST09_16515) - 3186177..3186470 (-) 294 WP_008348267.1 competence protein ComFB -
  NST09_RS16520 (NST09_16520) comFA 3186535..3187662 (-) 1128 WP_234898350.1 DEAD/DEAH box helicase Machinery gene
  NST09_RS16525 (NST09_16525) - 3188034..3188876 (-) 843 WP_144683538.1 DegV family protein -
  NST09_RS16530 (NST09_16530) degU 3189088..3189777 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  NST09_RS16535 (NST09_16535) degS 3189842..3191017 (-) 1176 WP_003213166.1 sensor histidine kinase Regulator
  NST09_RS16540 (NST09_16540) - 3191239..3191907 (+) 669 WP_017357860.1 YigZ family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42889.85 Da        Isoelectric Point: 10.4871

>NTDB_id=983700 NST09_RS16520 WP_234898350.1 3186535..3187662(-) (comFA) [Bacillus sp. FSL W7-1346]
MLGKVSECGFLYEWTGPQKKDTRQVELTWQGELSKGQKRASDRLVEAIQNKFDLLVWAVCGAGKTEVLFHGIKYALNNGM
SVCIATPRTDVVLELEPRMRKAFQGLNIAALYGGSSQRFQMAPLMIATTHQLMRYKNAFDVLIVDEVDAFPYSIDERLQF
AVLKSMKKTGVRIYLSATPSKKMQKDVARRQLEAIKIPLRFHQKPLPVPTFQWVGDWKKRLKRNRLPASTMNWLHEHITK
KKRVLLFVPSISTMKKVTHILQGQDVNVQGVSADDQERKQKVQHFRDHRYDVLVTTTILERGVTIQDVQVGVLGAESPIF
TESALVQISGRAGRHPDFFNGDVYFFHFGLTRSMKQAKKHIVKMNNTAEKEFSEK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=983700 NST09_RS16520 WP_234898350.1 3186535..3187662(-) (comFA) [Bacillus sp. FSL W7-1346]
ATGCTGGGAAAAGTAAGCGAATGTGGCTTTCTCTACGAATGGACGGGTCCACAAAAGAAAGACACAAGACAAGTTGAATT
AACATGGCAGGGGGAGCTTTCTAAAGGGCAAAAGAGAGCGTCCGATAGGCTAGTAGAAGCAATCCAAAACAAATTTGATC
TGCTCGTTTGGGCTGTTTGTGGTGCGGGGAAAACGGAGGTGCTTTTTCATGGAATAAAATATGCGCTAAATAACGGAATG
AGTGTTTGTATTGCGACGCCTAGAACCGATGTTGTACTTGAGCTTGAACCTCGAATGAGAAAGGCATTTCAAGGACTGAA
TATAGCTGCACTTTATGGCGGGAGTTCTCAAAGATTTCAAATGGCGCCGCTTATGATCGCTACAACCCATCAGCTAATGA
GATATAAAAACGCCTTTGATGTCCTCATTGTAGACGAAGTCGATGCTTTTCCTTATTCAATCGATGAACGGCTTCAGTTT
GCTGTTTTAAAGTCAATGAAAAAAACAGGAGTTAGAATTTACTTAAGTGCCACACCATCTAAAAAAATGCAAAAAGATGT
TGCTCGTAGACAATTAGAAGCGATCAAAATTCCTCTGCGCTTCCACCAAAAACCGTTACCTGTCCCAACATTTCAATGGG
TTGGAGATTGGAAAAAGAGATTAAAAAGAAATCGGCTTCCAGCATCTACAATGAACTGGCTGCATGAACATATCACAAAG
AAAAAAAGAGTGTTACTCTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACTCATATTCTTCAAGGTCAAGATGTAAA
TGTACAGGGTGTTTCTGCGGATGATCAAGAAAGGAAGCAAAAAGTCCAGCATTTCAGAGATCATAGATACGATGTATTGG
TTACAACAACTATTCTAGAAAGAGGCGTTACCATTCAAGATGTTCAAGTGGGCGTCTTAGGTGCGGAATCTCCTATTTTT
ACAGAAAGTGCACTTGTTCAAATTTCTGGTAGAGCAGGGAGACATCCAGATTTTTTTAACGGAGACGTGTACTTTTTTCA
TTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAACATATCGTAAAAATGAATAATACAGCTGAAAAAGAGTTTTCTG
AAAAATAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(32-180)

Helicase conserved C-terminal domain (Helicase_C)

(236-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

55.946

98.667

0.552
  comFA/cflA Streptococcus pneumoniae Rx1

41.243

94.4

0.389
  comFA/cflA Streptococcus pneumoniae D39

41.243

94.4

0.389
  comFA/cflA Streptococcus pneumoniae R6

41.243

94.4

0.389
  comFA/cflA Streptococcus pneumoniae TIGR4

40.96

94.4

0.387
  comFA/cflA Streptococcus mitis NCTC 12261

40.678

94.4

0.384
  comFA Latilactobacillus sakei subsp. sakei 23K

38.315

98.133

0.376
  comFA/cflA Streptococcus mitis SK321

39.548

94.4

0.373
  comFA Lactococcus lactis subsp. cremoris KW2

37.432

97.6

0.365

Multiple sequence alignment