Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   NST24_RS11625 Genome accession   NZ_CP151977
Coordinates   2268739..2269032 (-) Length   97 a.a.
NCBI ID   WP_008342186.1    Uniprot ID   A0A5K1NAM4
Organism   Bacillus sp. FSL R5-0286     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2263739..2274032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST24_RS11575 (NST24_11575) sinR 2263903..2264238 (+) 336 WP_017357978.1 helix-turn-helix domain-containing protein Regulator
  NST24_RS11580 (NST24_11580) - 2264306..2265106 (-) 801 WP_008342195.1 TasA family protein -
  NST24_RS11585 (NST24_11585) - 2265165..2265737 (-) 573 WP_017357979.1 signal peptidase I -
  NST24_RS11590 (NST24_11590) tapA 2265787..2266314 (-) 528 WP_342461795.1 amyloid fiber anchoring/assembly protein TapA -
  NST24_RS11595 (NST24_11595) - 2266567..2266893 (+) 327 WP_342461796.1 DUF3889 domain-containing protein -
  NST24_RS11600 (NST24_11600) - 2266928..2267122 (-) 195 WP_008342191.1 YqzE family protein -
  NST24_RS11605 (NST24_11605) comGG 2267183..2267566 (-) 384 WP_251211210.1 competence type IV pilus minor pilin ComGG -
  NST24_RS11610 (NST24_11610) comGF 2267563..2268060 (-) 498 WP_216894677.1 competence type IV pilus minor pilin ComGF -
  NST24_RS11615 (NST24_11615) comGE 2268002..2268316 (-) 315 WP_047945462.1 competence type IV pilus minor pilin ComGE -
  NST24_RS11620 (NST24_11620) comGD 2268300..2268746 (-) 447 WP_342461797.1 competence type IV pilus minor pilin ComGD -
  NST24_RS11625 (NST24_11625) comGC 2268739..2269032 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  NST24_RS11630 (NST24_11630) comGB 2269049..2270089 (-) 1041 WP_342461798.1 competence type IV pilus assembly protein ComGB -
  NST24_RS11635 (NST24_11635) comGA 2270070..2271140 (-) 1071 WP_024718839.1 competence type IV pilus ATPase ComGA Machinery gene
  NST24_RS11640 (NST24_11640) - 2271599..2272471 (-) 873 WP_035390819.1 STAS domain-containing protein -
  NST24_RS11650 (NST24_11650) - 2272697..2273077 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NST24_RS11655 (NST24_11655) - 2273290..2273532 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10728.62 Da        Isoelectric Point: 6.7081

>NTDB_id=983034 NST24_RS11625 WP_008342186.1 2268739..2269032(-) (comGC) [Bacillus sp. FSL R5-0286]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQSIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIFIQNGDVKNE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=983034 NST24_RS11625 WP_008342186.1 2268739..2269032(-) (comGC) [Bacillus sp. FSL R5-0286]
ATGAATGATAAAGGATTTACACTGTTAGAAATGTTAATTGTCATGCTTGTGATCTCCATTCTTTTATTGATCACCATTCC
AAATATCACTCGGCATCATCAAAGCATTCAAGCAAAGGGCTGTGAAGGGCTGAAATCAATGGTGAGTACACAGGTAATGG
CATATGAATTAGAGCATGACGGCAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGCTATGTCAAAAAAGGATTAACT
TGTCCGAATGGAAAGGCAATTTTCATTCAAAACGGCGATGTGAAAAATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5K1NAM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67
  comGC Staphylococcus aureus MW2

41.304

94.845

0.392
  comGC Staphylococcus aureus N315

41.304

94.845

0.392

Multiple sequence alignment