Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NST24_RS11635 Genome accession   NZ_CP151977
Coordinates   2270070..2271140 (-) Length   356 a.a.
NCBI ID   WP_024718839.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0286     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2265070..2276140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST24_RS11585 (NST24_11585) - 2265165..2265737 (-) 573 WP_017357979.1 signal peptidase I -
  NST24_RS11590 (NST24_11590) tapA 2265787..2266314 (-) 528 WP_342461795.1 amyloid fiber anchoring/assembly protein TapA -
  NST24_RS11595 (NST24_11595) - 2266567..2266893 (+) 327 WP_342461796.1 DUF3889 domain-containing protein -
  NST24_RS11600 (NST24_11600) - 2266928..2267122 (-) 195 WP_008342191.1 YqzE family protein -
  NST24_RS11605 (NST24_11605) comGG 2267183..2267566 (-) 384 WP_251211210.1 competence type IV pilus minor pilin ComGG -
  NST24_RS11610 (NST24_11610) comGF 2267563..2268060 (-) 498 WP_216894677.1 competence type IV pilus minor pilin ComGF -
  NST24_RS11615 (NST24_11615) comGE 2268002..2268316 (-) 315 WP_047945462.1 competence type IV pilus minor pilin ComGE -
  NST24_RS11620 (NST24_11620) comGD 2268300..2268746 (-) 447 WP_342461797.1 competence type IV pilus minor pilin ComGD -
  NST24_RS11625 (NST24_11625) comGC 2268739..2269032 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  NST24_RS11630 (NST24_11630) comGB 2269049..2270089 (-) 1041 WP_342461798.1 competence type IV pilus assembly protein ComGB -
  NST24_RS11635 (NST24_11635) comGA 2270070..2271140 (-) 1071 WP_024718839.1 competence type IV pilus ATPase ComGA Machinery gene
  NST24_RS11640 (NST24_11640) - 2271599..2272471 (-) 873 WP_035390819.1 STAS domain-containing protein -
  NST24_RS11650 (NST24_11650) - 2272697..2273077 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NST24_RS11655 (NST24_11655) - 2273290..2273532 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  NST24_RS11660 (NST24_11660) - 2273581..2274219 (-) 639 WP_202734344.1 MBL fold metallo-hydrolase -
  NST24_RS11665 (NST24_11665) - 2274380..2274553 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  NST24_RS11670 (NST24_11670) - 2274578..2274892 (-) 315 WP_017367597.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40366.72 Da        Isoelectric Point: 9.0449

>NTDB_id=983035 NST24_RS11635 WP_024718839.1 2270070..2271140(-) (comGA) [Bacillus sp. FSL R5-0286]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEANL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=983035 NST24_RS11635 WP_024718839.1 2270070..2271140(-) (comGA) [Bacillus sp. FSL R5-0286]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGATCATTGGCT
GTGATGTTAAGACGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCAACTGTAAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACAGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGGAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCCAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACATTGCAC
GCAAAGAATGCAAAAGGAGCTCTTTATCGAATGCTCGAATTTGGTGTGACGATGAATGAACTCGAACAAACAATGGTTGC
TATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGGTTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAACCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.888

100

0.649

Multiple sequence alignment