Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   NSS78_RS16895 Genome accession   NZ_CP151974
Coordinates   3273085..3274212 (-) Length   375 a.a.
NCBI ID   WP_335353006.1    Uniprot ID   -
Organism   Bacillus sp. FSL W8-0920     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3268085..3279212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSS78_RS16860 (NSS78_16860) flgL 3268265..3269176 (-) 912 WP_137054345.1 flagellar hook-associated protein FlgL -
  NSS78_RS16865 (NSS78_16865) flgK 3269186..3270706 (-) 1521 WP_003213544.1 flagellar hook-associated protein FlgK -
  NSS78_RS16870 (NSS78_16870) - 3270722..3271204 (-) 483 WP_050944342.1 flagellar protein FlgN -
  NSS78_RS16875 (NSS78_16875) flgM 3271223..3271486 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  NSS78_RS16880 (NSS78_16880) - 3271566..3271985 (-) 420 WP_003212865.1 TIGR03826 family flagellar region protein -
  NSS78_RS16885 (NSS78_16885) comFC 3272042..3272728 (-) 687 WP_212045588.1 ComF family protein Machinery gene
  NSS78_RS16890 (NSS78_16890) - 3272725..3273018 (-) 294 WP_050944343.1 competence protein ComFB -
  NSS78_RS16895 (NSS78_16895) comFA 3273085..3274212 (-) 1128 WP_335353006.1 DEAD/DEAH box helicase Machinery gene
  NSS78_RS16900 (NSS78_16900) - 3274584..3275426 (-) 843 WP_003213402.1 DegV family protein -
  NSS78_RS16905 (NSS78_16905) degU 3275644..3276333 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  NSS78_RS16910 (NSS78_16910) degS 3276398..3277573 (-) 1176 WP_003213166.1 sensor histidine kinase Regulator
  NSS78_RS16915 (NSS78_16915) - 3277795..3278463 (+) 669 WP_003212936.1 YigZ family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42882.28 Da        Isoelectric Point: 10.5170

>NTDB_id=982869 NSS78_RS16895 WP_335353006.1 3273085..3274212(-) (comFA) [Bacillus sp. FSL W8-0920]
MMGKTTECGFLYEWTGPQMEEACHSELTWQGNLSKGQKRASEKLIEAIKNKSDLLIWAVCGAGKTEVLFHGIEYALHQGM
SVCIATPRTDVVLELEPRLKKAFQGMKIAVLYGGSPQRFQIEKLVIATTHQLMRYKHAFDVLIIDEVDAFPYSIDGRLQF
AVLKAMRKKGVRVYLSATPSKKMIRDVSRGQLEAIKIPLRFHQQPLPVPSFQWIGHWKKKLKKNQLPPKVMNWMQKHITK
KRRVLLFVPSISTMKKVTKVLRKHHLNVEGVSADDPDRKQKVQQFRDYKYDVLVTTTILERGVTISNVQVGVLGAESTIF
TESALVQISGRAGRHPDFFKGDVFFFHFGLTRSMKQAKKHIVKMNDTAEKEFSEK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=982869 NSS78_RS16895 WP_335353006.1 3273085..3274212(-) (comFA) [Bacillus sp. FSL W8-0920]
ATGATGGGGAAAACAACGGAATGTGGCTTTCTTTATGAATGGACGGGTCCACAAATGGAAGAAGCATGTCACTCGGAATT
AACATGGCAGGGAAACCTGTCGAAAGGACAAAAAAGAGCGTCAGAGAAATTGATCGAAGCGATAAAAAACAAATCTGATC
TACTCATCTGGGCAGTTTGCGGGGCAGGGAAAACAGAGGTGCTTTTTCACGGAATAGAATATGCCTTACATCAAGGAATG
AGTGTCTGCATTGCGACACCTAGAACTGATGTTGTCCTTGAACTAGAGCCGCGGCTTAAAAAAGCTTTTCAAGGAATGAA
AATAGCTGTTCTTTATGGAGGAAGTCCTCAAAGGTTCCAAATAGAAAAGTTAGTAATCGCTACAACTCACCAGTTGATGA
GATATAAACATGCATTTGATGTGCTCATTATAGATGAAGTCGACGCCTTCCCTTATTCAATCGATGGACGTCTCCAATTT
GCTGTTTTAAAGGCAATGAGAAAGAAAGGGGTTAGGGTTTATTTAAGTGCGACGCCGTCTAAGAAAATGATAAGAGATGT
ATCTCGTGGACAACTAGAAGCCATAAAAATTCCTCTGCGCTTTCACCAACAACCTTTACCGGTCCCATCCTTTCAATGGA
TTGGACATTGGAAGAAGAAATTAAAAAAGAATCAGCTCCCACCTAAAGTAATGAATTGGATGCAGAAACATATTACAAAA
AAGAGAAGGGTTTTACTTTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACGAAGGTTCTTAGAAAGCACCATTTAAA
TGTAGAAGGGGTGTCTGCTGATGATCCAGATAGGAAACAAAAGGTCCAGCAATTCAGAGATTACAAATACGATGTACTAG
TTACAACCACAATTCTAGAAAGAGGTGTAACCATTTCAAATGTGCAAGTGGGGGTACTAGGTGCAGAATCTACTATCTTT
ACAGAAAGTGCACTTGTTCAGATTTCTGGTAGAGCAGGTAGACATCCCGATTTTTTTAAAGGAGATGTTTTCTTTTTTCA
TTTTGGTTTAACGAGAAGTATGAAGCAAGCAAAGAAGCATATTGTAAAAATGAATGATACAGCTGAAAAAGAGTTCTCCG
AAAAATAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(236-335)

Type III restriction enzyme, res subunit (ResIII)

(32-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

58.108

98.667

0.573
  comFA Latilactobacillus sakei subsp. sakei 23K

39.247

99.2

0.389
  comFA/cflA Streptococcus mitis SK321

40.395

94.4

0.381
  comFA/cflA Streptococcus pneumoniae Rx1

39.831

94.4

0.376
  comFA/cflA Streptococcus pneumoniae D39

39.831

94.4

0.376
  comFA/cflA Streptococcus pneumoniae R6

39.831

94.4

0.376
  comFA/cflA Streptococcus pneumoniae TIGR4

39.548

94.4

0.373
  comFA/cflA Streptococcus mitis NCTC 12261

39.266

94.4

0.371
  comFA Lactococcus lactis subsp. cremoris KW2

37.883

95.733

0.363

Multiple sequence alignment