Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   AAGA51_RS01215 Genome accession   NZ_CP151842
Coordinates   269751..270350 (+) Length   199 a.a.
NCBI ID   WP_342291518.1    Uniprot ID   -
Organism   Vibrio diazotrophicus strain ATCC 33466     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 264751..275350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGA51_RS01200 - 265551..268028 (-) 2478 WP_042485078.1 penicillin-binding protein 1A -
  AAGA51_RS01205 pilM 268171..269181 (+) 1011 WP_042485081.1 type IV pilus assembly protein PilM Machinery gene
  AAGA51_RS01210 pilN 269171..269758 (+) 588 WP_042485084.1 PilN domain-containing protein Machinery gene
  AAGA51_RS01215 pilO 269751..270350 (+) 600 WP_342291518.1 type 4a pilus biogenesis protein PilO Machinery gene
  AAGA51_RS01220 pilP 270347..270862 (+) 516 WP_042485090.1 pilus assembly protein PilP Machinery gene
  AAGA51_RS01225 pilQ 270881..272626 (+) 1746 WP_042485093.1 type IV pilus secretin PilQ Machinery gene
  AAGA51_RS01230 aroK 272813..273331 (+) 519 WP_042485096.1 shikimate kinase AroK -
  AAGA51_RS01235 aroB 273359..274444 (+) 1086 WP_042485100.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 23069.40 Da        Isoelectric Point: 5.2867

>NTDB_id=981074 AAGA51_RS01215 WP_342291518.1 269751..270350(+) (pilO) [Vibrio diazotrophicus strain ATCC 33466]
MGDFKNLELDEILDWPRLPQLVVLLLVVVLIQGAGYWFYLLPKQEHLSALIEEEQTLKSTIRIKAHKAATLPQLKAQLDE
LSERYDYLLQQLPEQKELASMLAAVNELGIKHQLSFNRMDWGEKEQQAFLYRLPLNLELTGEYHHIGQFSQAIAQLPRII
YFEHVDFQRASQDSQKLSVNVRAFTYQYQPDSLTSGGQQ

Nucleotide


Download         Length: 600 bp        

>NTDB_id=981074 AAGA51_RS01215 WP_342291518.1 269751..270350(+) (pilO) [Vibrio diazotrophicus strain ATCC 33466]
ATGGGTGATTTTAAAAACCTAGAGCTTGACGAAATATTGGATTGGCCACGATTGCCACAGCTCGTGGTGTTGCTACTTGT
CGTGGTGCTGATTCAAGGTGCGGGATATTGGTTTTATCTTTTGCCCAAGCAAGAGCATTTGAGCGCGCTCATTGAAGAAG
AACAAACGCTAAAGTCCACCATTCGAATTAAAGCGCATAAAGCTGCAACTCTGCCGCAACTCAAAGCTCAACTCGATGAA
CTCTCAGAACGTTATGACTATTTGTTACAGCAACTACCCGAGCAGAAAGAGCTGGCCAGTATGTTGGCGGCGGTGAATGA
GTTGGGCATCAAACATCAACTGAGTTTCAATCGGATGGATTGGGGTGAAAAAGAACAACAAGCATTTTTATATCGCCTGC
CTTTGAATCTTGAACTGACAGGTGAATATCACCATATTGGCCAGTTTTCACAAGCGATTGCACAGTTGCCAAGGATTATC
TATTTCGAGCACGTAGACTTTCAGCGAGCCAGTCAAGACAGTCAGAAATTGAGCGTCAATGTACGAGCCTTTACCTATCA
GTATCAGCCGGATTCGCTAACCTCAGGAGGTCAACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(49-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

66.138

94.975

0.628
  pilO Vibrio campbellii strain DS40M4

64.211

95.477

0.613

Multiple sequence alignment