Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AAGA51_RS01205 Genome accession   NZ_CP151842
Coordinates   268171..269181 (+) Length   336 a.a.
NCBI ID   WP_042485081.1    Uniprot ID   -
Organism   Vibrio diazotrophicus strain ATCC 33466     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 263171..274181
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGA51_RS01190 - 264201..264455 (-) 255 WP_042485071.1 DUF3624 domain-containing protein -
  AAGA51_RS01195 oxyR 264593..265486 (+) 894 WP_042485075.1 DNA-binding transcriptional regulator OxyR -
  AAGA51_RS01200 - 265551..268028 (-) 2478 WP_042485078.1 penicillin-binding protein 1A -
  AAGA51_RS01205 pilM 268171..269181 (+) 1011 WP_042485081.1 type IV pilus assembly protein PilM Machinery gene
  AAGA51_RS01210 pilN 269171..269758 (+) 588 WP_042485084.1 PilN domain-containing protein Machinery gene
  AAGA51_RS01215 pilO 269751..270350 (+) 600 WP_342291518.1 type 4a pilus biogenesis protein PilO Machinery gene
  AAGA51_RS01220 pilP 270347..270862 (+) 516 WP_042485090.1 pilus assembly protein PilP Machinery gene
  AAGA51_RS01225 pilQ 270881..272626 (+) 1746 WP_042485093.1 type IV pilus secretin PilQ Machinery gene
  AAGA51_RS01230 aroK 272813..273331 (+) 519 WP_042485096.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37112.70 Da        Isoelectric Point: 6.8337

>NTDB_id=981072 AAGA51_RS01205 WP_042485081.1 268171..269181(+) (pilM) [Vibrio diazotrophicus strain ATCC 33466]
MVQSLVMGVDIGHHSIKAVVLKPAGSKYTLLGYKEIRIADDIFADNHALVYQKIVKKLKELKKGLPLFSRKVVIAIPDNT
VISKVLQIDSELEPNEREFAIHQAFSHQSPYLMEEVSLDFIPVIKHPSPETKTSQNNIAAYQVYATKRDVVESRTVALEK
AGFKPILAEVQTHALVQLWQLFSKTYARTDWLLVDVGHTQTTLCLDFANKAPFYKQISEGVSQVSTQSENEDSVVIKDNV
NELVEKIAKQIQLFISVNGAASLTGIWLTGGGANIDAIQSALTEKVDLMCGVLDPFSVFHDKASKRYRSELPVAAFATAA
GLAARGINWLEVEHAA

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=981072 AAGA51_RS01205 WP_042485081.1 268171..269181(+) (pilM) [Vibrio diazotrophicus strain ATCC 33466]
ATGGTTCAATCATTAGTTATGGGTGTCGATATCGGCCACCATAGTATTAAAGCAGTGGTCCTAAAACCTGCAGGCAGTAA
GTATACGCTGTTAGGTTATAAGGAAATACGAATTGCTGACGATATTTTCGCTGATAACCATGCCTTGGTTTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGTTTACCTTTATTTAGTCGAAAAGTTGTTATTGCCATCCCCGATAACACC
GTAATTAGTAAAGTATTACAAATAGATAGTGAGCTTGAACCTAATGAACGCGAGTTCGCTATTCATCAAGCGTTTTCTCA
TCAATCTCCTTACCTGATGGAAGAGGTCAGTCTGGATTTTATTCCAGTCATAAAGCATCCATCGCCTGAAACAAAGACTA
GCCAAAATAATATAGCAGCCTACCAAGTGTATGCCACTAAGCGAGACGTTGTAGAGAGCCGTACAGTGGCTTTGGAAAAA
GCGGGTTTTAAGCCGATTCTGGCTGAAGTGCAAACCCATGCTTTAGTTCAGTTGTGGCAACTGTTCAGCAAAACTTATGC
ACGCACTGATTGGTTGCTGGTGGATGTCGGTCATACCCAAACCACCTTGTGCTTAGATTTTGCCAATAAAGCACCTTTTT
ATAAGCAGATTTCAGAAGGTGTTTCTCAGGTATCGACTCAATCAGAAAATGAAGATTCTGTAGTAATAAAAGACAACGTC
AATGAGCTGGTGGAGAAGATCGCCAAGCAAATTCAGTTGTTCATATCGGTGAACGGTGCGGCAAGTCTTACCGGAATCTG
GTTAACCGGTGGTGGGGCAAATATCGACGCGATACAAAGCGCGTTGACAGAGAAGGTCGATCTCATGTGCGGCGTGTTAG
ACCCATTTAGCGTATTCCATGACAAAGCGAGCAAGCGTTACCGTTCTGAATTGCCTGTAGCGGCTTTTGCCACAGCGGCA
GGACTTGCCGCACGCGGCATCAATTGGTTGGAGGTTGAACATGCTGCATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-205)

Type IV pilus assembly protein PilM; (PilM_2)

(229-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

55.685

100

0.568
  pilM Vibrio campbellii strain DS40M4

41.935

100

0.426

Multiple sequence alignment