Detailed information NTG0980138

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AAFA28_RS15655
• Genome accession: NZ_CP151699
• Coordinates: 3243245..3244363 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain F19-10-9
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3238245..3249363

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAFA28_RS15645 (AAFA28_15645)	-	3241361..3242053 (-)	693	WP_001108513.1	YggS family pyridoxal phosphate-dependent enzyme	-
AAFA28_RS15650 (AAFA28_15650)	pilT	3242180..3243217 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
AAFA28_RS15655 (AAFA28_15655)	pilU	3243245..3244363 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AAFA28_RS15660 (AAFA28_15660)	fur	3244433..3244870 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
AAFA28_RS15665 (AAFA28_15665)	bamE	3244982..3245380 (+)	399	WP_001170993.1	outer membrane protein assembly factor BamE	-
AAFA28_RS15670 (AAFA28_15670)	-	3245435..3245758 (-)	324	WP_000442582.1	RnfH family protein	-
AAFA28_RS15675 (AAFA28_15675)	-	3245758..3246825 (-)	1068	WP_004736014.1	hypothetical protein	-
AAFA28_RS15680 (AAFA28_15680)	-	3246978..3247433 (-)	456	WP_000782976.1	bacteriohemerythrin	-
AAFA28_RS15685 (AAFA28_15685)	-	3247685..3248533 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=980138 AAFA28_RS15655 WP_000347039.1 3243245..3244363(+) (pilU) [Acinetobacter baumannii strain F19-10-9]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=980138 AAFA28_RS15655 WP_000347039.1 3243245..3244363(+) (pilU) [Acinetobacter baumannii strain F19-10-9]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTACAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCGCTTAAACATGCTGATTCACCGAACGATTTGCG
TTTAACCATTAAACTTGCAGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395