Detailed information NTG0980032

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AAFA12_RS03800
• Genome accession: NZ_CP151696
• Coordinates: 757235..758353 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain F19-10-MH12
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 752235..763353

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAFA12_RS03765 (AAFA12_03765)	-	753117..753242 (+)	126	Protein_714	hemerythrin	-
AAFA12_RS03770 (AAFA12_03770)	-	753288..754220 (-)	933	WP_000743272.1	IS5-like element IS17 family transposase	-
AAFA12_RS03775 (AAFA12_03775)	-	754276..754620 (+)	345	Protein_716	bacteriohemerythrin	-
AAFA12_RS03780 (AAFA12_03780)	-	754773..755840 (+)	1068	WP_004736014.1	hypothetical protein	-
AAFA12_RS03785 (AAFA12_03785)	-	755840..756163 (+)	324	WP_000442582.1	RnfH family protein	-
AAFA12_RS03790 (AAFA12_03790)	bamE	756218..756616 (-)	399	WP_001170993.1	outer membrane protein assembly factor BamE	-
AAFA12_RS03795 (AAFA12_03795)	fur	756728..757165 (+)	438	WP_342068187.1	ferric iron uptake transcriptional regulator	-
AAFA12_RS03800 (AAFA12_03800)	pilU	757235..758353 (-)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AAFA12_RS03805 (AAFA12_03805)	pilT	758381..759418 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
AAFA12_RS03810 (AAFA12_03810)	-	759545..760237 (+)	693	WP_001108513.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=980032 AAFA12_RS03800 WP_000347039.1 757235..758353(-) (pilU) [Acinetobacter baumannii strain F19-10-MH12]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=980032 AAFA12_RS03800 WP_000347039.1 757235..758353(-) (pilU) [Acinetobacter baumannii strain F19-10-MH12]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTACAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCGCTTAAACATGCTGATTCACCGAACGATTTGCG
TTTAACCATTAAACTTGCAGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395