Detailed information NTG0979907

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AAFA13_RS15335
• Genome accession: NZ_CP151690
• Coordinates: 3214115..3215233 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain F19-10-CH8
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3209115..3220233

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAFA13_RS15325 (AAFA13_15325)	-	3212231..3212923 (-)	693	WP_001108513.1	YggS family pyridoxal phosphate-dependent enzyme	-
AAFA13_RS15330 (AAFA13_15330)	pilT	3213050..3214087 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
AAFA13_RS15335 (AAFA13_15335)	pilU	3214115..3215233 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AAFA13_RS15340 (AAFA13_15340)	fur	3215303..3215740 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
AAFA13_RS15345 (AAFA13_15345)	bamE	3215852..3216250 (+)	399	WP_001170993.1	outer membrane protein assembly factor BamE	-
AAFA13_RS15350 (AAFA13_15350)	-	3216305..3216628 (-)	324	WP_000442582.1	RnfH family protein	-
AAFA13_RS15355 (AAFA13_15355)	-	3216628..3217695 (-)	1068	WP_004736014.1	hypothetical protein	-
AAFA13_RS15360 (AAFA13_15360)	-	3217848..3218303 (-)	456	WP_000782976.1	bacteriohemerythrin	-
AAFA13_RS15365 (AAFA13_15365)	-	3218555..3219403 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=979907 AAFA13_RS15335 WP_000347039.1 3214115..3215233(+) (pilU) [Acinetobacter baumannii strain F19-10-CH8]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=979907 AAFA13_RS15335 WP_000347039.1 3214115..3215233(+) (pilU) [Acinetobacter baumannii strain F19-10-CH8]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTACAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCGCTTAAACATGCTGATTCACCGAACGATTTGCG
TTTAACCATTAAACTTGCAGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395