Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   ACNHKU_RS20495 Genome accession   NZ_CP182142
Coordinates   4134616..4135854 (-) Length   412 a.a.
NCBI ID   WP_000815980.1    Uniprot ID   -
Organism   Escherichia coli strain CUPF09     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4129616..4140854
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNHKU_RS20475 (ACNHKU_20475) dam 4130228..4131064 (-) 837 WP_000742143.1 adenine-specific DNA-methyltransferase -
  ACNHKU_RS20480 (ACNHKU_20480) damX 4131171..4132457 (-) 1287 WP_094336956.1 cell division protein DamX -
  ACNHKU_RS20485 (ACNHKU_20485) aroB 4132549..4133637 (-) 1089 WP_000439850.1 3-dehydroquinate synthase -
  ACNHKU_RS20490 (ACNHKU_20490) aroK 4133694..4134215 (-) 522 WP_000818618.1 shikimate kinase AroK -
  ACNHKU_RS20495 (ACNHKU_20495) comE 4134616..4135854 (-) 1239 WP_000815980.1 DNA uptake porin HofQ Machinery gene
  ACNHKU_RS20500 (ACNHKU_20500) hofP 4135766..4136170 (-) 405 WP_001338740.1 DNA utilization protein HofP -
  ACNHKU_RS20505 (ACNHKU_20505) hofO 4136160..4136600 (-) 441 WP_001055768.1 DNA utilization protein HofO -
  ACNHKU_RS20510 (ACNHKU_20510) hofN 4136584..4137123 (-) 540 WP_352452303.1 DNA utilization protein HofN -
  ACNHKU_RS20515 (ACNHKU_20515) hofM 4137123..4137902 (-) 780 WP_001338738.1 DNA utilization protein HofM -
  ACNHKU_RS20520 (ACNHKU_20520) mrcA 4138022..4140574 (+) 2553 WP_001300707.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44670.21 Da        Isoelectric Point: 6.3183

>NTDB_id=979612 ACNHKU_RS20495 WP_000815980.1 4134616..4135854(-) (comE) [Escherichia coli strain CUPF09]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNNNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGNDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPLFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=979612 ACNHKU_RS20495 WP_000815980.1 4134616..4135854(-) (comE) [Escherichia coli strain CUPF09]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAAGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGCCAGGAGGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATAACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCACTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAACGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGTTTGCTGGATCTTGAGCTTTCTGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCTAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCTGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTTGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  comE Haemophilus influenzae 86-028NP

36.927

100

0.391
  pilQ Vibrio campbellii strain DS40M4

37.767

100

0.386
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.53

100

0.376
  pilQ Vibrio cholerae strain A1552

37.53

100

0.376
  comE Glaesserella parasuis strain SC1401

36.19

100

0.369