Detailed information NTG0979471

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AAEY34_RS02040
• Genome accession: NZ_CP151647
• Coordinates: 441258..442406 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain HN148
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 436258..447406

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAEY34_RS02015 (AAEY34_02015)	-	437409..437705 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
AAEY34_RS02020 (AAEY34_02020)	-	437702..438295 (-)	594	WP_003084544.1	YggT family protein	-
AAEY34_RS02025 (AAEY34_02025)	proC	438306..439127 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
AAEY34_RS02030 (AAEY34_02030)	-	439139..439831 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
AAEY34_RS02035 (AAEY34_02035)	pilT	440046..441080 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
AAEY34_RS02040 (AAEY34_02040)	pilU	441258..442406 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
AAEY34_RS02045 (AAEY34_02045)	czcD	442414..443313 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
AAEY34_RS02050 (AAEY34_02050)	-	443467..443871 (+)	405	WP_003084560.1	NINE protein	-
AAEY34_RS02055 (AAEY34_02055)	-	444114..445487 (+)	1374	WP_203305983.1	pyridoxal-phosphate dependent enzyme	-
AAEY34_RS02060 (AAEY34_02060)	-	445484..446668 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=979471 AAEY34_RS02040 WP_003100959.1 441258..442406(+) (pilU) [Pseudomonas aeruginosa strain HN148]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=979471 AAEY34_RS02040 WP_003100959.1 441258..442406(+) (pilU) [Pseudomonas aeruginosa strain HN148]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382