Detailed information NTG0978996

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AACL51_RS23705
• Genome accession: NZ_CP151537
• Coordinates: 5038409..5039557 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PA98zyfcau
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5033409..5044557

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AACL51_RS23685 (AACL51_23685)	-	5034147..5035331 (-)	1185	WP_023086800.1	cystathionine gamma-synthase	-
AACL51_RS23690 (AACL51_23690)	-	5035328..5036701 (-)	1374	WP_015502220.1	pyridoxal-phosphate dependent enzyme	-
AACL51_RS23695 (AACL51_23695)	-	5036944..5037348 (-)	405	WP_003084560.1	NINE protein	-
AACL51_RS23700 (AACL51_23700)	czcD	5037502..5038401 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
AACL51_RS23705 (AACL51_23705)	pilU	5038409..5039557 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
AACL51_RS23710 (AACL51_23710)	pilT	5039735..5040769 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
AACL51_RS23715 (AACL51_23715)	-	5040984..5041676 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
AACL51_RS23720 (AACL51_23720)	proC	5041688..5042509 (+)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
AACL51_RS23725 (AACL51_23725)	-	5042520..5043113 (+)	594	WP_003084544.1	YggT family protein	-
AACL51_RS23730 (AACL51_23730)	-	5043110..5043406 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=978996 AACL51_RS23705 WP_003100959.1 5038409..5039557(-) (pilU) [Pseudomonas aeruginosa strain PA98zyfcau]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=978996 AACL51_RS23705 WP_003100959.1 5038409..5039557(-) (pilU) [Pseudomonas aeruginosa strain PA98zyfcau]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382