Detailed information NTG0097646

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: ACAM23_RS01585
• Genome accession: NZ_AP031394
• Coordinates: 276483..277481 (+)
• Length: 332 a.a.
• NCBI ID: WP_001809468.1
• Uniprot ID: Q8CWU7
• Organism: Streptococcus pneumoniae strain 16P28
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 271483..282481

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACAM23_RS01565 (SPNE16P28_03020)	-	273420..274262 (+)	843	WP_001081167.1	hypothetical protein	-
ACAM23_RS01570 (SPNE16P28_03030)	-	274318..275014 (+)	697	Protein_306	GNAT family N-acetyltransferase	-
ACAM23_RS01575 (SPNE16P28_03040)	-	275026..275442 (+)	417	WP_000628384.1	GNAT family N-acetyltransferase	-
ACAM23_RS01580	-	276257..276349 (-)	93	WP_000970381.1	type I toxin-antitoxin system Fst family toxin	-
ACAM23_RS01585 (SPNE16P28_03050)	ruvB	276483..277481 (+)	999	WP_001809468.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
ACAM23_RS01590 (SPNE16P28_03060)	-	277462..278034 (+)	573	WP_001098893.1	nucleotidyltransferase family protein	-
ACAM23_RS01595 (SPNE16P28_03070)	-	278265..279023 (+)	759	WP_000466719.1	isoprenyl transferase	-
ACAM23_RS01600 (SPNE16P28_03080)	-	279032..279835 (+)	804	WP_000189857.1	phosphatidate cytidylyltransferase	-
ACAM23_RS01605 (SPNE16P28_03090)	eeP	279857..281116 (+)	1260	WP_000900660.1	RIP metalloprotease RseP	Regulator

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37288.73 Da
• Isoelectric Point: 4.5053

>NTDB_id=97646 ACAM23_RS01585 WP_001809468.1 276483..277481(+) (ruvB) [Streptococcus pneumoniae strain 16P28]
MSRILDNEIMGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPVIEKAGDLVAILNELEPGDVLFIDEIHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRVRDFAQIMGNGVID
DIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSVNIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAK
AYEHLGYEYSEK

Nucleotide

• Length: 999 bp

>NTDB_id=97646 ACAM23_RS01585 WP_001809468.1 276483..277481(+) (ruvB) [Streptococcus pneumoniae strain 16P28]
ATGAGTAGAATTTTAGATAATGAGATAATGGGGGATGAGGAGTTAGTAGAACGCACGCTCCGTCCTCAGTATTTACGTGA
ATATATCGGACAGGATAAGGTCAAGGACCAGCTACAAATCTTTATTGAAGCTGCCAAAATGCGGGATGAAGCGCTGGATC
ATGTGCTCTTATTTGGGCCTCCAGGTCTCGGGAAAACGACCATGGCCTTTGTTATTGCCAACGAACTGGGAGTCAATCTT
AAGCAGACGTCGGGTCCAGTCATTGAAAAAGCCGGAGATCTGGTAGCTATTTTGAATGAGTTAGAGCCTGGGGATGTACT
TTTTATTGATGAGATCCATCGTTTGCCAATGTCAGTGGAAGAGGTGCTTTATAGTGCTATGGAGGACTTCTACATAGATA
TTATGATTGGGGCTGGTGAGGGTAGTCGTAGTGTTCATTTGGAGTTACCACCTTTTACCTTGATTGGTGCGACGACTCGG
GCTGGTATGCTCTCCAATCCGCTACGGGCACGTTTTGGGATTACAGGCCATATGGAGTATTATGCCCATGCTGACTTGAC
AGAAATTGTCGAGCGGACGGCAGATATTTTTGAGATGGAAATCACTCATGAGGCAGCATCTGAGTTGGCCCTACGTAGTC
GTGGGACCCCTCGTATTGCCAATCGTCTCCTCAAGCGCGTGCGCGATTTTGCCCAGATAATGGGGAATGGGGTAATTGAT
GATATTATTACCGATAAGGCTTTGACTATGCTGGATGTTGACCATGAAGGTTTGGACTATGTGGATCAAAAAATCCTTCG
TACCATGATTGAGATGTACAGTGGAGGACCTGTTGGTCTAGGAACTCTTTCTGTCAATATCGCCGAAGAGCGTGAGACAG
TTGAAGATATGTATGAGCCTTACTTGATTCAAAAAGGTTTTATCATGCGGACACGGTCTGGACGGGTGGCGACTGCTAAG
GCATATGAGCACTTAGGTTATGAATACAGTGAAAAATAA

Domains

Predicted by InterProScan.

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: Q8CWU7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	100	100	1
ruvB	Streptococcus pneumoniae D39	100	100	1
ruvB	Streptococcus pneumoniae TIGR4	99.096	100	0.991
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.429	98.193	0.593
ruvB	Helicobacter pylori 26695	50.774	97.289	0.494
ruvB	Synechocystis sp. PCC 6803	49.84	94.277	0.47