Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   ACNFID_RS01725 Genome accession   NZ_CP180702
Coordinates   378055..379293 (+) Length   412 a.a.
NCBI ID   WP_000815968.1    Uniprot ID   -
Organism   Escherichia coli strain FREC5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 373055..384293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNFID_RS01700 mrcA 373335..375887 (-) 2553 WP_416045046.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  ACNFID_RS01705 hofM 376007..376786 (+) 780 WP_001315880.1 DNA utilization protein HofM -
  ACNFID_RS01710 hofN 376786..377325 (+) 540 WP_001069315.1 DNA utilization protein HofN -
  ACNFID_RS01715 hofO 377309..377749 (+) 441 WP_089568420.1 DNA utilization protein HofO -
  ACNFID_RS01720 hofP 377739..378143 (+) 405 WP_001264141.1 DNA utilization protein HofP -
  ACNFID_RS01725 comE 378055..379293 (+) 1239 WP_000815968.1 DNA uptake porin HofQ Machinery gene
  ACNFID_RS01730 aroK 379694..380215 (+) 522 WP_000818618.1 shikimate kinase AroK -
  ACNFID_RS01735 aroB 380272..381360 (+) 1089 WP_000439850.1 3-dehydroquinate synthase -
  ACNFID_RS01740 damX 381452..382738 (+) 1287 WP_000343199.1 cell division protein DamX -
  ACNFID_RS01745 dam 382845..383681 (+) 837 WP_000742143.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44774.27 Da        Isoelectric Point: 6.1173

>NTDB_id=976334 ACNFID_RS01725 WP_000815968.1 378055..379293(+) (comE) [Escherichia coli strain FREC5]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEDNILSVHSIAWQNNNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=976334 ACNFID_RS01725 WP_000815968.1 378055..379293(+) (comE) [Escherichia coli strain FREC5]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGGCAGGAAGACAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATAACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGCTTGCTGGATCTTGAGCTTTCTGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.385

100

0.396
  comE Haemophilus influenzae 86-028NP

37.156

100

0.393
  pilQ Vibrio campbellii strain DS40M4

37.767

100

0.386
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.288

100

0.374
  pilQ Vibrio cholerae strain A1552

37.288

100

0.374
  comE Glaesserella parasuis strain SC1401

35.952

100

0.367