Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AADH33_RS01660 Genome accession   NZ_CP151283
Coordinates   393157..394212 (+) Length   351 a.a.
NCBI ID   WP_348549350.1    Uniprot ID   A0AAU6S1D3
Organism   Psychrobacter sp. KFRI-CH2-11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 388157..399212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AADH33_RS01650 (AADH33_01650) - 389375..389908 (+) 534 WP_075100329.1 DUF6586 family protein -
  AADH33_RS01655 (AADH33_01655) - 390344..392962 (-) 2619 WP_348549349.1 PBP1A family penicillin-binding protein -
  AADH33_RS01660 (AADH33_01660) pilM 393157..394212 (+) 1056 WP_348549350.1 pilus assembly protein PilM Machinery gene
  AADH33_RS01665 (AADH33_01665) - 394212..394862 (+) 651 WP_348549351.1 PilN domain-containing protein -
  AADH33_RS01670 (AADH33_01670) pilO 394859..395566 (+) 708 WP_075100333.1 type 4a pilus biogenesis protein PilO -
  AADH33_RS01675 (AADH33_01675) - 395563..396096 (+) 534 WP_075100334.1 pilus assembly protein PilP -
  AADH33_RS01680 (AADH33_01680) pilQ 396124..398508 (+) 2385 WP_348549352.1 type IV pilus secretin PilQ -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38279.50 Da        Isoelectric Point: 4.4364

>NTDB_id=976074 AADH33_RS01660 WP_348549350.1 393157..394212(+) (pilM) [Psychrobacter sp. KFRI-CH2-11]
MRLFTSKSRHLIGVDICATSVKLVDIQHQQGMFHLKSYGIERLPEGVVVDKLIVDTEAVGDTIASLARRCQVANAHAATA
VSGSAVITKIIDMDMALSDVEREAQIRLDADQYVPYPLEDVNLDFEVLGPSLVSDDMVQVLLAASRSENVDQRVDALTFG
GMQTKVMDIESHAIERAFGLMVDNLPGLPELVALVDIGHNQTTLYVAKNGEFIYSREQLFGGAQLTEAIQNRYGLSSEEA
ALSKRERTLPDDYYPEILTPFIENTIQQITRSLQFYFSSSQYSNIDHVVLAGGSSSIPGLAGMAQQKLGVTVSIANPFTN
MTIAPHIDNEQLAIDAPSLMAACGLALRSFD

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=976074 AADH33_RS01660 WP_348549350.1 393157..394212(+) (pilM) [Psychrobacter sp. KFRI-CH2-11]
GTGAGGCTATTTACTTCCAAAAGTCGACATTTGATTGGAGTGGATATCTGTGCCACCTCAGTAAAGTTGGTTGACATACA
GCACCAACAAGGCATGTTCCACTTAAAATCTTATGGCATTGAAAGACTACCGGAAGGCGTAGTCGTCGATAAACTCATCG
TGGATACTGAAGCCGTTGGCGATACGATTGCCAGTTTGGCCAGACGCTGTCAAGTGGCTAATGCCCATGCAGCGACAGCT
GTATCAGGGTCAGCGGTGATTACCAAGATTATCGATATGGACATGGCACTCAGCGATGTCGAGCGCGAAGCGCAGATACG
TCTCGATGCCGATCAATACGTGCCTTACCCTCTTGAAGACGTCAACCTAGACTTTGAAGTCTTAGGCCCTTCTTTGGTCA
GTGACGATATGGTGCAGGTGCTACTGGCGGCCTCACGCTCGGAGAATGTCGACCAACGCGTCGATGCCTTAACCTTCGGC
GGCATGCAAACCAAGGTCATGGATATTGAGTCGCACGCTATTGAGCGTGCTTTTGGTTTGATGGTAGATAATTTACCAGG
GCTACCAGAATTAGTCGCTTTGGTAGATATTGGTCACAATCAAACCACCTTATATGTCGCCAAAAATGGCGAATTTATCT
ATAGCCGCGAACAGCTATTTGGCGGTGCTCAGCTTACAGAGGCAATACAAAATCGCTATGGCTTATCGTCTGAGGAAGCG
GCGCTGAGTAAGCGTGAGCGCACCTTGCCTGACGATTACTATCCTGAGATATTGACGCCGTTTATCGAAAACACCATTCA
GCAAATCACTCGCTCTTTGCAGTTCTATTTTTCTTCGAGCCAATACAGCAATATCGATCACGTGGTCCTCGCGGGGGGCA
GTAGCTCGATTCCCGGTCTGGCTGGCATGGCGCAACAAAAACTGGGCGTGACTGTCAGTATCGCCAATCCATTTACCAAT
ATGACCATCGCGCCACATATCGATAATGAGCAACTGGCGATCGATGCCCCAAGCCTCATGGCAGCATGTGGTCTTGCGTT
AAGGAGCTTTGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-350)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.695

100

0.521
  comM Acinetobacter nosocomialis M2

51.695

100

0.521
  comM Acinetobacter baylyi ADP1

48.87

100

0.493
  pilM Legionella pneumophila strain ERS1305867

40.51

100

0.407

Multiple sequence alignment