Detailed information NTG0975639

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V5E96_RS11205
• Genome accession: NZ_CP151175
• Coordinates: 2403423..2404571 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 3860P
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2398423..2409571

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V5E96_RS11185 (V5E96_011185)	-	2399161..2400345 (-)	1185	WP_003100953.1	cystathionine gamma-synthase	-
V5E96_RS11190 (V5E96_011190)	-	2400342..2401715 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
V5E96_RS11195 (V5E96_011195)	-	2401958..2402362 (-)	405	WP_003084560.1	NINE protein	-
V5E96_RS11200 (V5E96_011200)	czcD	2402516..2403415 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
V5E96_RS11205 (V5E96_011205)	pilU	2403423..2404571 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
V5E96_RS11210 (V5E96_011210)	pilT	2404749..2405783 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
V5E96_RS11215 (V5E96_011215)	-	2405998..2406690 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
V5E96_RS11220 (V5E96_011220)	proC	2406702..2407523 (+)	822	WP_003114890.1	pyrroline-5-carboxylate reductase	-
V5E96_RS11225 (V5E96_011225)	-	2407534..2408127 (+)	594	WP_003084544.1	YggT family protein	-
V5E96_RS11230 (V5E96_011230)	-	2408124..2408420 (+)	297	WP_031655952.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=975639 V5E96_RS11205 WP_003100959.1 2403423..2404571(-) (pilU) [Pseudomonas aeruginosa strain 3860P]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=975639 V5E96_RS11205 WP_003100959.1 2403423..2404571(-) (pilU) [Pseudomonas aeruginosa strain 3860P]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382