Detailed information NTG0097505

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: ABXH20_RS08500
• Genome accession: NZ_AP031328
• Coordinates: 1563244..1564218 (+)
• Length: 324 a.a.
• NCBI ID: WP_011227913.1
• Uniprot ID: Q5SL87
• Organism: Thermus thermophilus strain TF24
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1558244..1569218

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABXH20_RS08475 (TthTF24_16710)	lysN	1558741..1559934 (+)	1194	WP_353300170.1	2-aminoadipate transaminase	-
ABXH20_RS08480	-	1559917..1560276 (-)	360	WP_353386005.1	hypothetical protein	-
ABXH20_RS08485 (TthTF24_16730)	-	1560569..1561462 (-)	894	WP_353300172.1	site-specific DNA-methyltransferase	-
ABXH20_RS08490 (TthTF24_16740)	-	1561459..1562385 (-)	927	WP_014630113.1	RluA family pseudouridine synthase	-
ABXH20_RS08495 (TthTF24_16750)	panB	1562433..1563218 (-)	786	WP_353300173.1	3-methyl-2-oxobutanoate hydroxymethyltransferase	-
ABXH20_RS08500 (TthTF24_16760)	ruvB	1563244..1564218 (+)	975	WP_011227913.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
ABXH20_RS08505 (TthTF24_16770)	-	1564215..1565246 (+)	1032	WP_143584725.1	DUF4388 domain-containing protein	-
ABXH20_RS08510 (TthTF24_16780)	-	1565267..1566619 (-)	1353	WP_008631640.1	tetratricopeptide repeat protein	-
ABXH20_RS08515 (TthTF24_16790)	ftsH	1566692..1568548 (+)	1857	WP_143584724.1	ATP-dependent zinc metalloprotease FtsH	-
ABXH20_RS08520 (TthTF24_16800)	-	1568534..1568962 (-)	429	WP_011227910.1	hypothetical protein	-

Sequence

Protein

• Length: 324 a.a.
• Molecular weight: 35973.74 Da
• Isoelectric Point: 6.3232

>NTDB_id=97505 ABXH20_RS08500 WP_011227913.1 1563244..1564218(+) (ruvB) [Thermus thermophilus strain TF24]
MEDLALRPKTLDEYIGQERLKQKLRVYLEAAKARKEPLEHLLLFGPPGLGKTTLAHVIAHELGVNLRVTSGPAIEKPGDL
AAILANSLEEGDILFIDEIHRLSRQAEEHLYPAMEDFVMDIVIGQGPAARTIRLELPRFTLIGATTRPGLITAPLLSRFG
IVEHLEYYTPEELAQGVMRDARLLGVRITEEAALEIGRRSRGTMRVAKRLFRRVRDFAQVAGEEVITRERALEALAALGL
DELGLEKRDREILEVLILRFGGGPVGLATLATALSEDPGTLEEVHEPYLIRQGLLKRTPRGRVATELAYRHLGYPPPVGP
LLEP

Nucleotide

• Length: 975 bp

>NTDB_id=97505 ABXH20_RS08500 WP_011227913.1 1563244..1564218(+) (ruvB) [Thermus thermophilus strain TF24]
GTGGAAGACCTCGCCCTTAGGCCCAAGACCCTGGACGAGTACATCGGCCAGGAACGCCTGAAGCAAAAGCTCCGGGTCTA
CCTCGAGGCGGCCAAGGCCCGAAAAGAGCCCTTGGAGCACCTCCTCCTCTTCGGCCCCCCGGGCCTGGGCAAGACCACCC
TGGCCCACGTCATCGCCCACGAGCTTGGGGTCAACCTCCGGGTCACCTCCGGGCCCGCCATAGAGAAGCCCGGGGACCTC
GCCGCCATCCTGGCCAACTCCCTGGAGGAAGGGGACATCCTCTTCATTGACGAGATCCACCGCCTGAGCCGCCAGGCCGA
GGAGCACCTCTACCCCGCCATGGAGGACTTCGTCATGGACATCGTCATCGGCCAAGGTCCGGCGGCGAGGACCATCCGGC
TGGAGCTTCCCCGCTTCACCCTGATCGGGGCCACCACCCGGCCCGGCCTCATCACCGCGCCCCTCTTGAGCCGCTTCGGC
ATCGTGGAGCACCTGGAGTACTACACCCCCGAGGAGCTGGCCCAAGGGGTGATGCGGGACGCCCGCCTCCTCGGGGTGAG
GATCACGGAGGAGGCCGCCCTGGAGATCGGTAGGCGGAGCCGGGGCACCATGCGCGTCGCCAAGCGCCTCTTCCGGAGGG
TGCGGGACTTCGCCCAGGTGGCGGGGGAGGAGGTCATCACCCGGGAGCGGGCCCTGGAGGCCCTTGCGGCCCTGGGCCTG
GACGAGCTGGGCTTGGAGAAGCGGGACCGGGAGATCCTGGAGGTCCTCATCCTCCGCTTCGGCGGTGGCCCAGTGGGCCT
CGCCACCTTAGCCACCGCCCTCTCCGAGGACCCGGGCACCTTGGAAGAGGTGCACGAGCCCTACCTCATCCGCCAGGGCC
TTTTGAAGCGCACTCCCCGGGGCCGGGTGGCCACGGAACTGGCCTACCGCCACCTGGGCTACCCGCCCCCGGTGGGCCCC
CTCTTGGAGCCGTGA

Domains

Predicted by InterProScan.

• RuvB AAA lid domain (AAA_lid_4) (168-241)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (6-165)

• RuvB C-terminal winged helix domain (WHD_RuvB) (242-312)

3D structure

• PDB: 1HQC
• PDB: 1IXR
• PDB: 1IXS
• PDB: 8EFV
• PDB: 8EFY

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	59.105	96.605	0.571
ruvB	Streptococcus pneumoniae TIGR4	56.774	95.679	0.543
ruvB	Streptococcus pneumoniae R6	56.774	95.679	0.543
ruvB	Streptococcus pneumoniae D39	56.774	95.679	0.543
ruvB	Synechocystis sp. PCC 6803	50.779	99.074	0.503
ruvB	Helicobacter pylori 26695	50.637	96.914	0.491