Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilV   Type   Machinery gene
Locus tag   ABXH20_RS04250 Genome accession   NZ_AP031328
Coordinates   760694..761044 (-) Length   116 a.a.
NCBI ID   WP_353300790.1    Uniprot ID   -
Organism   Thermus thermophilus strain TF24     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 755694..766044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABXH20_RS04215 (TthTF24_08270) trpS 756530..757543 (+) 1014 WP_018461708.1 tryptophan--tRNA ligase -
  ABXH20_RS04220 (TthTF24_08280) - 757540..758196 (+) 657 WP_353300786.1 chromosome segregation protein ScpA -
  ABXH20_RS04225 (TthTF24_08290) - 758215..758772 (+) 558 WP_124104344.1 Uma2 family endonuclease -
  ABXH20_RS04230 (TthTF24_08300) - 758806..759168 (-) 363 WP_060384345.1 HepT-like ribonuclease domain-containing protein -
  ABXH20_RS04235 (TthTF24_08310) - 759155..759454 (-) 300 WP_008631315.1 nucleotidyltransferase -
  ABXH20_RS04240 (TthTF24_08320) - 759477..760019 (-) 543 WP_353300788.1 Uma2 family endonuclease -
  ABXH20_RS04245 (TthTF24_08330) - 760140..760445 (-) 306 WP_353300789.1 DUF1640 domain-containing protein -
  ABXH20_RS04250 (TthTF24_08340) pilV 760694..761044 (-) 351 WP_353300790.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ABXH20_RS04255 (TthTF24_08350) comZ 761275..762948 (-) 1674 WP_353300791.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  ABXH20_RS04260 (TthTF24_08360) pilA/pilA3 762959..763660 (-) 702 WP_014629427.1 PilW family protein Machinery gene
  ABXH20_RS04265 (TthTF24_08370) pilA/pilA2 763657..764238 (-) 582 WP_353300792.1 type II secretion system protein Machinery gene
  ABXH20_RS04270 (TthTF24_08380) pilA/pilA1 764273..764743 (-) 471 WP_165739610.1 GspH/FimT family protein Machinery gene
  ABXH20_RS04275 (TthTF24_08390) - 764909..765889 (+) 981 WP_353300793.1 YpdA family putative bacillithiol disulfide reductase -

Sequence

Protein


Download         Length: 116 a.a.        Molecular weight: 12174.83 Da        Isoelectric Point: 3.9798

>NTDB_id=97488 ABXH20_RS04250 WP_353300790.1 760694..761044(-) (pilV) [Thermus thermophilus strain TF24]
MRNAKGFTLIELLIVIAIIAILAAVLIPNVLNARKRATDSAAQAFGRQVITWLAAADTAGVDVTEVTSCTDELLQAEGAP
AELPNGVAECSIEYDGAGQWTVTVTSDAGNTFTFTY

Nucleotide


Download         Length: 351 bp        

>NTDB_id=97488 ABXH20_RS04250 WP_353300790.1 760694..761044(-) (pilV) [Thermus thermophilus strain TF24]
ATGCGGAACGCGAAAGGTTTCACCCTGATTGAGCTCCTGATCGTCATCGCCATCATCGCCATCCTGGCGGCGGTGCTGAT
CCCGAACGTCTTGAACGCCCGCAAGCGGGCCACCGATAGCGCCGCTCAGGCCTTTGGCCGCCAAGTCATCACCTGGCTCG
CCGCCGCTGACACGGCCGGTGTGGACGTCACTGAGGTCACGAGCTGTACCGACGAGCTCCTTCAAGCGGAAGGGGCTCCC
GCTGAACTCCCCAATGGGGTAGCTGAGTGCTCCATCGAGTACGATGGCGCCGGTCAGTGGACGGTGACGGTCACGTCTGA
TGCCGGTAACACGTTCACCTTCACTTACTGA

Domains

Predicted by InterProScan.

Pilin A4 (PilA4)

(39-114)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.966

100

0.44
  pilA2 Legionella pneumophila str. Paris

41.739

99.138

0.414
  pilA2 Legionella pneumophila strain ERS1305867

40.87

99.138

0.405

Multiple sequence alignment